Simple Western antibody database

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The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Charge- and Size-based assays. This database is intended to provide assay general development guidance in identifying and selecting antibodies to test. The performance of any antibody selected will need to be optimized for the test system being examined.

Check out other antibodies that have been screened and tested with Simple Western Charge- and Size-based assays! You can sort by column headings or just type into the Search box.

If you don't see your antibody listed here, shoot us an email at support@proteinsimple.com.

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Antibodies (2886)

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Protein Target	Antibody Type	Vendor	Product Number	Protein Isoform	Species	Cell Model(s)	Separated by Size or Charge	Dilution	kDa on Simple Western	Matrix
14-3-3 γ	Primary	Novus Biologicals	NBP2-27202	14-3-3 gamma (γ)	Rabbit polyclonal	Human Brain lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:50	33 kDa	12-230 kDa
14-3-3 γ	Primary	Novus Biologicals	NBP2-27370	14-3-3 gamma (γ)	Rabbit Polyclonal	Human Brain lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:10	34 kDa	12-230 kDa
14-3-3 γ	Primary	Novus Biologicals	NB100-406	14-3-3 gamma (γ)	Mouse Monoclonal	HeLa lysate 1.0 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:12.5	36 kDa	12-230 kDa
14-3-3 ζ	Primary	R&D Systems	MAB2669	14-3-3 zeta (ζ)	Mouse Monoclonal	SH-SY5Y human neuroblastoma cell line	Size-Jess/Wes, Sally Sue/Peggy Sue	2 µg/mL	34 kDa	12-230 kDa
15-PGDH/HPGD	Primary	Novus Biologicals	NB200-179	15-PGDH/HPGD	Rabbit polyclonal	Lovo (left) and HeLa (right) lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:500		12-230kD
15-PGDH/HPGD	Primary	R&D Systems	AF5660	15-PGDH/HPGD	Goat Polyclonal	Human liver tissue	Size-Jess/Wes, Sally Sue/Peggy Sue	10 µg/mL	34 kDa	12-230kD
17 beta-HSD1/HSD17B1	Primary	R&D Systems	MAB7178	17 beta-HSD1/HSD17B1	Mouse Monoclonal	Human placenta tissue	Size-Jess/Wes, Sally Sue/Peggy Sue	2.5 µg/mL	40 kDa	12-230kD
4-Hydroxynonenal	Primary	R&D Systems	MAB3249	4-Hydroxynonenal	Mouse Monoclonal	HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE)	Size-Jess/Wes, Sally Sue/Peggy Sue	5 µg/mL		12-230kD
4EBP1	Primary	R&D Systems	AF3227	4EBP1	Goat Polyclonal	HeLa human cervical epithelial carcinoma cell line	Size	5 µg/mL	26 kDa	12-230kD
5'-Nucleotidase/CD73	Primary	R&D Systems	AF5795	5'-Nucleotidase/CD73	Sheep Polyclonal	U266 human myeloma cell line	Size-Jess/Wes, Sally Sue/Peggy Sue	10 µg/mL	86	12-230 kDa
5-Lipoxygenase	Primary	Novus Biologicals	NB110-58748	5-Lipoxygenase	Rabbit polyclonal	Human Lung lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	50 µg/ml		12-230kD
5-Lipoxygenase	Primary	Novus Biologicals	NB110-58749	5-Lipoxygenase	Rabbit polyclonal	Human Lung lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	10 µg/ml		12-230kD
5HT1D	Primary	Novus Biologicals	NB100-56349	5HT1D	Rabbit polyclonal	Human Brain lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:10		12-230kD
5HT3A	Primary	Novus Biologicals	NB100-56351	5HT3A	Rabbit polyclonal	Human Brain lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:50	65	12-230kD
6-His Epitope Tag	Primary	Novus Biologicals	NB100-64768	6-His Epitope Tag	Mouse monoclonal	rhEDG6 CHO S Tx lysate 0.1 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:250		12-230kD
A20/TNFAIP3	Primary	Novus Biologicals	NBP1-77533	A20/TNFAIP3	Mouse monoclonal	Jurkat lysate 0.05 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:100	102	12-230kD
A2BP1	Primary	Novus Biologicals	NBP2-13169	A2BP1	Mouse monoclonal	Human Brain lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:20	54	12-230kD
ABCA1	Primary	Novus Biologicals	NB400-105	ABCA1	Rabbit polyclonal		Charge	1:50
ABCF2	Primary	Novus Biologicals	NB400-115	ABCF2	Rabbit polyclonal	HeLa lysate 0.05 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:1000	77	12-230kD
ABCG2	Primary	Novus Biologicals	NBP1-59749	ABCG2	Rabbit polyclonal	HepG2 lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:50	72	12-230kD
Abhd5	Primary	Novus Biologicals	NB110-41576	Abhd5	Rabbit polyclonal	MCF-7 lysate 0.5 mg/ml	Size-Jess/Wes, Sally Sue/Peggy Sue	1:1000	55	12-230kD
ACE-2	Primary	R&D Systems	AF3437	ACE-2	Goat Polyclonal	Mouse kidney tissue	Size-Jess/Wes, Sally Sue/Peggy Sue	2.5 µg/mL	141	12-230 kDa
ACE-2	Primary	R&D Systems	AF933	ACE-2	Goat Polyclonal	Human kidney tissue	Size-Jess/Wes, Sally Sue/Peggy Sue	10 µg/mL	155	12-230 kDa
ACE/CD143	Primary	R&D Systems	AF1513	ACE/CD143	Goat Polyclonal	Mouse lung tissue	Size-Jess/Wes, Sally Sue/Peggy Sue	10 µg/mL	218	66-440kD
Acrp30	Primary (biotinylated)	R&D Systems	BAF1065	Acrp30	Goat	Human Serum	Size-Jess/Wes, Sally Sue/Peggy Sue	1:50	40	12-230kD

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