CE-SDS with Maurice
CE-SDS (capillary electrophoresis sodium dodecyl sulfate) is an indispensable tool for characterization of biotherapeutic proteins and confirmation of product purity, and Maurice makes it automated, reliable, and easy. He transforms traditional CE-SDS to provide the highest quality data with a vastly simplified workflow, to let you quickly and effectively monitor critical quality attributes (CQAs) like purity, identity, consistency, and stability, so you can get safe and effective products to market, faster.
Read on to learn more:
CE-SDS on Maurice Just Got Better
We’ve made it even easier to transition from SDS-PAGE to CE-SDS on Maurice by adding the new Lane View to our Compass for iCE software. Lane View gives you a gel-like representation of your CE-SDS data, giving you a way to visualize your data the way you’re used to with SDS-PAGE.
Odors from reducing agents like β-mercaptoethanol (BME) clearing out your lab? Our new System Filter Upgrade adds hardware to Maurice and Maurice S. to filter out odors after your CE-SDS runs on Maurice and Maurice S, so your lab stays stink-free. Request a Quote to learn more!
Advantages of CE-SDS Over SDS-PAGE
The accurate and quantitative analysis of product purity is a fundamental component of effective development of a biotherapeutic protein. Without consistent techniques and high-quality, reproducible results, it is difficult to precisely assess the product’s efficacy and or safety, potentially delaying, even jeopardizing, its approval. Hear about the advantages of automated CE-SDS on Maurice over manual SDS-PAGE by listening to our Podcast with Drug Discovery World (DDW) and Dr. Tufan Aydogdu, Field Applications Scientist, ProteinSimple, a Bio-Techne brand.
Are you still using SDS-PAGE for your protein analysis? When CE-SDS and SDS-PAGE methods are compared side-by-side, CE-SDS offers several clear advantages such as automation, improved quantitation, reproducibility, and faster sample turn-around time. Learn how Maurice CE-SDS reigns supreme against traditional SDS-PAGE slab-gels in this Application Note – Comparing SDS-Page with Maurice CE-SDS, and see how you can trust Maurice to provide reproducible, high-resolution data with a simple and robust workflow.
Molecular Weight Determination on CE-SDS and SDS-Page
Differences in apparent molecular weight (MW) have been observed in some cases when going from SDS-PAGE to CE-SDS. While these methods aren’t typically intended for high-resolution MW determination, many of their applications require a reasonable estimate of apparent MW. This Technical Note highlights relevant peer-reviewed publications that address factors for comparing MW between CE-SDS and SDS-PAGE.
Researchers at the University of Braunschweig evaluated the comparability of molecular weight determination of proteins on SDS-PAGE and CE-SDS on Maurice and Simple Western. In this paper, they focused on sample preparation and MW marker selection and their impact on apparent molecular weight.
CE-SDS Instruments and Techniques Are Not All Created Equal
Superior data quality and ease-of-use
You have a lot of choices for CE-SDS platforms; why choose Maurice? In this publication in the Journal Electrophoresis, scientists at Technical University of Braunschweig compared Maurice CE-SDS against several other instruments, and concluded that Maurice demonstrates superior data quality in all four parameters, with additional advantages in ease-of-use, flexibility, reliability and sustainability.
See how Maurice stands out
Maurice combines high data quality and a simplified workflow to give you superior confidence in your protein sample identity and purity, so that you can be sure to meet regulatory guidelines, while getting your product to market quickly. Check out these two Application Notes that show how CE-SDS analysis on Maurice of a NISTmAb (RM 8671) ranks against alternative instruments including the PA 800/PA 800 Plus system from SCIEX and the LabChip GXII Touch system from PerkinElmer.
Develop your biosimilars faster
If you are looking for tools to evaluate your biosimilars according to ICH Q6B guidelines, this white paper is for you. You’ll discover how to accelerate your biosimilar development using an automated CE-SDS platform that affords excellent reproducibility and ease-of-use, thus enabling faster comparability studies for monoclonal antibodies, heavily glycosylated molecules, and PEGylated species. Read white paper – Use of Maurice CE-SDS in ICH Q6B Based Biosimilar Comparability Exercises
Power up Maurice with Waters™ Empower® Software
Maurice gives you the data integrity, repeatability, and accuracy you need in your regulated laboratory environment. With a seamless integration with Waters™ Empower® 3 Software, the Maurice Empower Control Kit controls Maurice to provide regulatory compliance readiness, including 21 CFR Part 11 controls, for industry-leading security and data integrity. Check out our Application Note on Powering Up Maurice with Waters™ Empower® Software to learn more.
Easily transfer your CE-SDS methods to Maurice
It’s easy to transfer methods from other CE-SDS systems to Maurice. With Maurice, you have flexibility with adjustment of injection time, separation time, separation voltage, and data analysis. Learn how scientists from Pfizer optimized their Maurice CE-SDS method to achieve comparable results to those of a conventional CE system in this Webinar on the Evaluation and Application of Maurice for Biopharmaceutical Analysis. Want to find out more about how you can transfer your CE-SDS method to Maurice? We can help. Contact us at info@proteinsimple.com.
Enhanced Analysis For QC Testing
QC-specific workflows made easy
With Maurice, you can develop a QC-specific workflow that eliminates the need to use a commercial internal standard, freeing you from potential concerns like introducing contaminants to test samples that might interfere with subsequent analyses. We show you how he does it in this Application Note - Application of Maurice CE-SDS for Biopharmaceutical QC Workflows.
Save time and money with Maurice for QC
Learn from this whitepaper for how Maurice’s simplified and streamlined CE-SDS workflow for QC provides substantial savings, in both time and money, compared to alternative instruments that are error-prone due to their complicated designs and laborious workflows.
Power up Maurice with Waters™ Empower® Software
Maurice gives you the data integrity, repeatability, and accuracy you need in your regulated laboratory environment. With a seamless integration with Waters™ Empower® 3 Software, the Maurice Empower Control Kit controls Maurice to provide regulatory compliance readiness, including 21 CFR Part 11 controls, for industry-leading security and data integrity. Check out our Application Note on Powering Up Maurice with Waters™ Empower® Software to learn more.
Take Protein Analysis to the Next Level with CE-SDS PLUS
Save on costs and get even better data with CE-SDS PLUS
Learn how Maurice’s CE-SDS PLUS system provides unparalleled sample stability and data consistency in this Application Note - Enhanced CE-SDS Analysis with Maurice’s CE-SDS PLUS System, so you can analyze protein samples that are susceptible to fragmentation with confidence. Also enjoy 60% reduced cost due to the significantly increased injection number per cartridge, compared to our standard CE-SDS cartridge.
Automated AAV characterization with low sample volumes
CE-SDS PLUS can help you tackle novel and challenging molecules such as adeno-associated virus (AAV), commonly used in gene therapy. See how Maurice with CE-SDS PLUS provides rapid and well-resolved product characterization of AAV capsid protein purity, with low sample volume requirements, in this Application Note – Characterization of Adeno-Associated Viral Vector Proteins Using Maurice CE-SDS.
