iCE Kits and Cartridges

iCE consumables make operating your system fast and easy - and get you to more consistent data at the same time. Our kits help you develop methods and run system qualifications, and come with all the reagents you need to simplify day-to-day operation. GMP/GLP needs? All consumables are tested and validated to meet them!

Please contact us at orders@proteinsimple.com for pricing and order information. iCE system kits and reagents expire 12 months after the date of manufacture.

NEW!   Introducing the iCE Method Development Kit

The iCE Method Development Kit is a one-stop shop for all your method development needs, including a test mix to make sure your system is ready to go. It includes all sample preparation reagents and a wide range of ampholytes, pI markers and additives that’ll let you tackle any protein. The kit can be used with both the FC and HT cartridges. Order yours today!

Cartridges

The new HT Cartridge cuts your run times down to 10 minutes, gives you comparable results to the FC Cartridge, and you won't need methyl cellulose in your sample mixtures. With the original FC Cartridge, you'll need methyl cellulose in both the rinse and sample solutions.

Part # Description Qty/Amt
101701 FC Cartridge (original cIEF cartridge). This cartridge has a 50 mm, 100 µm I.D. fluorocarbon-coated capillary and built-in electrolyte tanks. 2/pkg
P-0000035-00 HT Cartridge. This cartridge has a 50 mm, 100 µm I.D. coated capillary and built-in electrolyte tanks. 2/pkg

Note: The HT Cartridge was developed and validated for the iCE3 only; we don't recommend using it on the iCE280.

Method Development Kit

Method development is just quicker and easier with the iCE Method Development Kit! You'll get a Method Development Guide plus all the reagents you need to analyze proteins on iCE systems. You can use this kit with either the FC or the HT Cartridge.

Part # Description
042-848 Method Development Kit. This kit includes a test mix, anolyte, catholyte, Methyl Cellulose, five types of ampholytes (Pharmalyte pH ranges 3-10, 2.5-5, 5-8 and 8-10.5 and Servalyte pH range 2-9), eight pI markers (3.21, 5.12, 6.14, 7.05, 7.65, 8.18, 9.46 and 10.10) and additives (urea and arginine).

Chemical Test Kits

These kits let you confirm the overall performance of your iCE system, and you can use the kit three different times. Kits are available for both the FC and HT cartridges.

Part # Description
101801 FC Cartridge Chemical Test Kit. This kit includes anolyte, catholyte, 0.5% Methyl Cellulose, a hemoglobin stock solution and three vials of a standard solution (carrier ampholytes and pI markers in 0.35% Methyl Cellulose).
P-0000033-00 HT Cartridge Chemical Test Kit. This kit includes anolyte, catholyte, 0.5% Methyl Cellulose, a hemoglobin stock solution and three vials of a standard solution (carrier ampholytes and pI markers in deionized water).

Electrolyte Kit

Simplify your system setup with our pre-made analytical grade electrolyte solutions. You can use this kit with either the FC or the HT Cartridge.

Part # Description
102506 Electrolyte Kit. This kit includes 1 x 100 mL bottle of 0.08 M phosphoric acid in 0.1% Methyl Cellulose and 1 x 100 mL bottle of 0.1 M sodium hydroxide in 0.1% Methyl Cellulose.

0.5% Methyl Cellulose Kit

0.5% Methyl Cellulose is needed to rinse the capillary between injections and minimize electroosmotic flow. You can use this kit with either the FC or the HT Cartridge.

Part # Description
102505 0.5% Methyl Cellulose Kit. This kit includes 2 x 100 mL bottles, which we viscosity test so your runs are consistent every time.

System Suitability Kits

These kits give you a handy way to check the performance of your iCE system up to 10 different times. Once you've prepared the test solutions, they're good for three days. Kits are available for both the FC and HT cartridges.

Part # Description
102093 FC Cartridge System Suitability Kit. This kit includes a hemoglobin stock solution and 10 vials of a standard solution (carrier ampholytes and pI markers in 0.35% Methyl Cellulose).
P-0000034-00 HT Cartridge System Suitability Kit. This kit includes a hemoglobin stock solution and 10 vials of a standard solution (carrier ampholytes and pI markers in deionized water).

1% Methyl Cellulose Kit

1% Methyl Cellulose is needed to maintain the viscosity of your samples on the FC Cartridge only.

Part # Description
101876 1% Methyl Cellulose Kit. This kit includes a 100 mL bottle, which we test so your runs are consistent every time.

TTM Solution Kits

Accurate transfer time measurements just give you more reproducible data, and reduce the amount of sample you use too. Use the kit for approximately 150 measurements. Kits are available for both the FC and HT cartridges.

Part # Description
102672 FC Cartridge Transfer Time Measurement Kit. This kit includes 2 x 8 mL bottles of TTM solution for the FC Cartridge.
P-0000037-00 HT Cartridge Transfer Time Measurement Kit. This kit includes 2 x 8 mL bottles of TTM solution for the HT Cartridge.

Cartridge Wash Kit

This kit lets you wash and store your FC or HT Cartridge the right way.

Part # Description
045-207 Cartridge Wash Kit. Includes step-by-step instructions on how to rinse and store your cartridges.

pI Markers

We have a wide range of markers for pI determination that will give you both consistent and accurate measurement of isoelectric point on your iCE system. They can be used for whole-column and single-point capillary isoelectric focusing (cIEF) detection to measure your sample's isoelectric point, and for quality control of sample analysis. You'll get 200 µL of pI marker stock solution in every vial.

Part # pI Value
102221 2.85
102018 3.21
102222 3.59
102350 4.22
102223 4.65
102224 5.12
102225 5.85
102220 6.14
102409 6.61
102226 7.05
102227 7.40
102170 7.55
102407 7.65
102171 7.90
102408 8.18
102229 8.40
102230 8.79
102231 9.22
102349 9.46
101996 9.50
102219 9.77
102232 10.10
102233 10.45