iCE Consumables and Reagents

Frequently Ordered Items

ProteinSimple provides a series of reagents and consumables for use with the iCE3 and iCE280 Systems. Please contact us at orders@proteinsimple.com for pricing and order information.

Part # 101701 cIEF Cartridge Kit

Part # Description Qty/Amt
101701 cIEF Cartridge 2/pkg

Part # 101801 Chemical Test Kit

The Chemical Test Kit is designed to confirm the overall performance of the iCE3 or iCE280 on 3 separate occasions. Each time, performance can be checked repeatedly over a 3 day period. The kit contains anolyte, catholyte, MC and a standard sample with Hemoglobin AFSC. Expiry dating is 12 months.

Solution # Description Qty/Amt
1 Anolyte, 0.08 M H3PO4 in 0.1% methyl cellulose (M.C.) 10 mL
2 Catholyte, 0.1% M.C. (NaOH added later) 5 mL X 3 vials
3 NaOH, 50% w/w (for Solution 2) 500 µL
4 0.5% methyl cellulose (MC) 10 mL
5 Hemoglobin (Hb stock) stock (for Solution 6) 30 µL
6 Standard sample with 8% pH 3-10 Pharmalytes, 2 pI Markers (4.22, 9.46) in 0.35% MC 1 mL X 3 vials
7 Deionized water in 1.5 ml vials 2 vials
8 0.5% MC Conditioning Solution 1 vial

Part #102506 Electrolyte Kit

These 100mL Anolyte and Catholyte solutions in 0.1% MC are used to fill the electrolyte tanks on the iCE3 and iCE280 cartridge. The labels and bottles are color coded to improve safety. The kit contains 2 x 100 mL bottles. Expiry dating is 12 months.

Solution # Description Qty/Amt
1 0.08M Phosphoric Acid (H3PO4) solution in 0.1% methyl cellulose 100 mL
2 0.1M Sodium Hydroxide (NAOH) solution in 0.1% methyl cellulose. 100 mL

Part #102505 0.5% Methyl Cellulose Kit

Use this concentration for the wash cycle between injections. The solution is filtered to ensure consistent viscosity to coat the capillary lumen to minimize electroosmotic flow (EOF). Conveniently packaged in 2 x 100mL bottles. Expiry dating is 12 months.

Solution # Description Qty/Amt
1 0.5% Methyl Cellulose 100 µL X 2 vials

Part #102093 System Suitability Kit

Use the System Suitability Kit to run a control test of the iCE3 or iCE280 on 10 separate occasions. Each time, the performance can be checked up to 6 times over 3 days. The kit contains a standard sample with Hemoglobin AFSC. Expiry dating is 12 months.

Solution # Description Qty/Amt
1 Hemoglobin stock 100 µL
2 Carrier Ampholyte Solution with 8% pH 3-10 Pharmalytes, 2 internal pI Markers (4.22, 9.46) in 0.35% Methyl Cellulose 0.5 mL X 10 vials

Part # 101876 1% Methyl Cellulose Kit

This solution is used to prepare samples. 100 ml bottle. Expiry dating is 12 months.

Solution # Description Qty/Amt
1 1% Methyl Cellulose Solution 100 mL

Part # 102672 TTM Solution Kit

The TTM Solution Kit (2 bottles X 8 mL) is used as the test solution in the iCE3 and iCE280 Transfer Time Measurement (TTM) routine performed in Cartridge Installation and Start Up procedures. This solution provides consistency and overcomes the problem of different definitions of the TTM plot's plateau height caused by using different test solutions. Expiry dating is 12 months.

Solution # Description Qty/Amt
1 TTM Solution 2 bottles X 8 mL

pI Markers

A new series of small molecule pI Markers with assigned pI values, designed to provide consistent and accurate results. The pI Markers may be used for whole-column and single-point capillary isoelectric focusing (cIEF) detection to measure a sample’s isoelectric point and to perform quality control of sample analysis. Each pI Marker comes in a vial with 200 µL of stock solution.

Part # pI Value
102221 2.85
102018 3.21
102222 3.59
102350 4.22
102223 4.65
102224 5.12
102225 5.85
102220 6.14
102409 6.61
102226 7.05
102227 7.40
102170 7.55
102407 7.65
102171 7.90
102408 8.18
102229 8.40
102230 8.79
102231 9.22
102349 9.46
101996 9.50
102219 9.77
102232 10.10
102233 10.45