NanoPro 100 reagents and consumables

NanoPro 100 reagents and consumables are designed for use on with NanoPro 100 and must be used in conjunction with Compass Software version 1.8 or higher and compatible firmware.

Assay Kits

Part # Description
CBS404
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HeLa Lysate Controls Kit - Bicine/CHAPS Buffer for Charge Assays
Includes 5 vials each of low phospho and high phospho HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (5 µL/vial). Ships overnight on dry ice. Upon receipt, store components at 4°C.
CBS403
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Lysis Kit - Bicine/CHAPS Buffer for Charge Assays
Includes Bicine/CHAPS Lysis Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix, and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C.
CBS401
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Lysis Kit - RIPA Buffer for Charge Assays
Includes RIPA Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C.
041-126
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Amplified Rabbit Secondary Antibody Detection Kit
Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Rabbit-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
041-127
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Amplified Mouse Secondary Antibody Detection Kit
Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Mouse-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
CBS3000
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NanoPro 100 Master Kit
Includes 1 box of eight capillary clips, eight NanoPro 100 assay plates, Anolyte, Catholyte, Wash Buffer, Luminol, and Peroxide. Ships overnight at room temperature. Upon receipt, store plates at room temperature, store capillaries in the dark at room temperature and desiccated, and all other components at 4°C.

Individual Reagents

Part # Description
040-655
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Goat-Anti-Mouse-HRP Secondary Antibody
HRP-conjugated Goat-Anti-Mouse Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-656
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Goat-Anti-Rabbit Secondary Antibody
HRP-conjugated Goat-Anti-Rabbit Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
041-081
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Goat-Anti-Rabbit HUX HRP Secondary Antibody
HRP-Conjugated Goat-Anti-Rabbit Secondary Antibody cross-absorbed for minimal cross-reactivity against human antigens (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-474
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Pan Erk Primary Antibody
Pan Erk Primary Antibody, 1X stock (1 mL)
040-477
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Phospho-Erk 1/2 Primary Antibody
Phospho-Erk 1/2 Primary Antibody, 1X stock (1 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-644
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pI Standard Ladder 1
pI Standard Ladder 1: 4.0, 4.9, 6.0, 6.4, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-645
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pI Standard Ladder 2
pI Standard Ladder 2: 4.2, 4.9, 6.0, 6.4, 7.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-646
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pI Standard Ladder 3
pI Standard Ladder 3: 4.9, 6.0, 6.4, 7.0, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-647
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pI Standard Ladder 4
pI Standard Ladder 4: 4.9, 5.5, 6.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-024
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pI Standard, 4.0
pI Standard, 4.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-025
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pI Standard, 4.2
pI Standard, 4.2 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-026
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pI Standard, 4.4
pI Standard, 4.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-027
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pI Standard, 4.9
pI Standard, 4.9 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-028
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pI Standard, 5.5
pI Standard, 5.5 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-029
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pI Standard, 6.0
pI Standard, 6.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-030
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pI Standard, 6.4
pI Standard, 6.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-031
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pI Standard, 7.0
pI Standard, 7.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-032
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pI Standard, 7.3
pI Standard, 7.3 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
041-036
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pI Standard, 8.4
pI Standard, 8.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-790
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pI Standard, 9.7
pI Standard, 9.7 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-967
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Premix G2, ampholyte-free separation gradient
Premix G2, ampholyte-free separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-968
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Premix G2, pH 3–10 separation gradient
Premix G2, pH 3–10 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-970
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Premix G2, pH 4–7 (nested) separation gradient
Premix G2, pH 4–7 (nested) separation gradient, pH 2-4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-969
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Premix G2, pH 4–9 separation gradient
Premix G2, pH 4–9 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-971
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Premix G2, pH 5–6 separation gradient
Premix G2, pH 5–6 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-972
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Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug
Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-973
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Premix G2, Pharmalyte pH 5–8 separation gradient
Premix G2, Pharmalyte pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-974
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Premix G2, Servalyt pH 5–8 separation gradient
Premix G2, Servalyt pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-337
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Anolyte Refill
Anolyte Refill (200 mL)
040-338
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Catholyte Refill
Catholyte Refill (200 mL)
041-108
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Wash Concentrate Refill for Peggy or NanoPro 1000
Wash Concentrate Refill (200 mL). REAGENT FOR PEGGY or NANOPRO 1000 USERS ONLY. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-652
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Luminol
Luminol (2 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-483
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RIPA Lysis Buffer
RIPA Lysis Buffer (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-764
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Bicine/CHAPS Lysis Buffer and Sample Diluent
Bicine/CHAPS Lysis Buffer and Sample Diluent (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-510
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DMSO Inhibitor Mix
DMSO Inhibitor Mix, 50X (200 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-482
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Aqueous Inhibitor Mix
Aqueous Inhibitor Mix, 25X (400 µL); Ships overnight on dry ice. Upon receipt, store at -20°C.
040-649
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Sample Diluent
Sample Diluent (3 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-309
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Antibody Diluent
Antibody Diluent (10 mL)
040-793
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Leveling Solution
NanoPro Leveling Solution

Consumables

Part # Description
CBS800
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NanoPro 100 Capillaries
1 box of 8 capillary clips. Ships overnight at room temperature. Upon receipt, store at room temperature. Light and moisture sensitive. When not in use keep box cover closed. Keep box desiccated.
040-901
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NanoPro 100 Plate Kit
Eight 36 well plates and lids. Ships overnight at room temperature. Upon receipt, store at room temperature.