Peggy reagents and consumables

Peggy reagents and consumables are designed for use on with Peggy and must be used in conjunction with Compass Software version 1.8 or higher and compatible firmware.

Size Separation Assay Kits

Part # Description
CBS-01-01
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Rabbit (12-180 kDa) Size Separation Master Kit for Sally or Peggy
Core Kit for Peggy or Sally, 5 boxes of Capillaries (96 capillaries per box), Size Separation Kit for Peggy or Sally, Size Detection Kit-Rabbit for Peggy or Sally, and Simple Western Standard 1 (MW ladder spans 12-180 kDa). Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS-02-01
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Mouse (12-180 kDa) Size Separation Master Kit for Sally or Peggy
Core Kit for Peggy or Sally, 5 boxes of Capillaries (96 capillaries per box), Size Separation Kit for Sally or Peggy, Size Detection Kit-Mouse for Peggy or Sally, and Simple Western Standard 1 (MW ladder spans 12-180 kDa). Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS301
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Core Kit for Sally or Peggy
Includes Matrix Removal Buffer, Wash Buffer, Running Buffer, Luminol-S, Peroxide, Running Buffer cup, Wash Buffer cup, Matrix Removal cup, 5 Manifold Cleaning Sponges and 5 Plates. Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS301S1
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Size Separation Kit for Sally or Peggy
Includes Separation Matrix, Stacking Matrix, 2 Fluorescent Standard vials, Standard Resuspension Buffer, Sample Buffer and 8 DTT vials. Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS301RD
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Size Detection Kit-Rabbit for Sally or Peggy
Goat-Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 480 Simple Western data points.
CBS301MD
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Size Detection Kit-Mouse for Sally or Peggy
Goat-Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 480 Simple Western data points.

Charge Separation Assay Kits

Part # Description
CBS2001
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XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000
Includes 5 boxes of capillaries, five 384-well plates with lids, Anolyte, Catholyte, Wash Concentrate, Luminol, and Peroxide XDR and manifold cleaning sponges.
CBS404
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HeLa Lysate Controls Kit - Bicine/CHAPS Buffer for Charge Assays
Includes 5 vials each of low phospho and high phospho HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (5 µL/vial). Ships overnight on dry ice. Upon receipt, store components at 4°C.
CBS403
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Lysis Kit - Bicine/CHAPS Buffer for Charge Assays
Includes Bicine/CHAPS Lysis Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix, and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C.
CBS401
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Lysis Kit - RIPA Buffer for Charge Assays
Includes RIPA Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C.
041-126
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Amplified Rabbit Secondary Antibody Detection Kit
Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Rabbit-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
041-127
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Amplified Mouse Secondary Antibody Detection Kit
Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Mouse-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
PS-T003
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Peggy System Charge Training Kit
Includes a XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 and additional reagents

Individual Reagents

Part # Description
CBS201STD1
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Simple Western Size Standard 1 for Sally, Peggy and Simon
Biotinylated Ladder 1 (MW ladder spans 12-180 kDa), Ladder Resuspension Buffer and Streptavidin-HRP. Reagents for 480 Simple Western data points.
041-695
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Single Use FL standard
Single Use FL standard-strip of 8 tubes (for use with Simple Western Size Standard 1 MW Ladder 12-180 kDa)
041-247
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Separation Matrix
Separation matrix 1 (2.5 mL)
041-248
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Stacking Matrix
Stacking matrix (1.5 mL)
041-265
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Simon, Sally, Peggy Sample Buffer
Simon, Sally, Peggy 10X Sample Buffer (400 µL)
041-808
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Antibody Diluent Plus
Antibody Diluent Plus for size assay (10 mL) for Simon, Sally or Peggy. Ships overnight at room temperature. Antibody Diluent is recommended for dilution of primary and secondary antibodies. Upon receipt, store at 4°C.
041-505
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Running Buffer for Sally or Peggy
Running Buffer (140 mL)
041-504
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Matrix Removal Buffer for Sally or Peggy
Matrix Removal Buffer (140 mL)
041-586
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Wash Buffer for Sally or Peggy
Wash Buffer (170 mL)
043-311
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Luminol-S
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Luminol-S (2 mL)
043-379
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Peroxide
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Peroxide (2 mL)
041-468
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HeLa Lysate Control - Bicine/CHAPS Buffer for Sally, Peggy or Simon
Includes 1 vial of HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (35 µL/vial)
040-655
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Goat-Anti-Mouse-HRP Secondary Antibody
HRP-conjugated Goat-Anti-Mouse Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-656
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Goat-Anti-Rabbit Secondary Antibody
HRP-conjugated Goat-Anti-Rabbit Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
041-081
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Goat-Anti-Rabbit HUX HRP Secondary Antibody
HRP-Conjugated Goat-Anti-Rabbit Secondary Antibody cross-absorbed for minimal cross-reactivity against human antigens (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-474
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Pan Erk Primary Antibody
Pan Erk Primary Antibody, 1X stock (1 mL)
040-477
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Phospho-Erk 1/2 Primary Antibody
Phospho-Erk 1/2 Primary Antibody, 1X stock (1 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-644
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pI Standard Ladder 1
pI Standard Ladder 1: 4.0, 4.9, 6.0, 6.4, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-645
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pI Standard Ladder 2
pI Standard Ladder 2: 4.2, 4.9, 6.0, 6.4, 7.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-646
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pI Standard Ladder 3
pI Standard Ladder 3: 4.9, 6.0, 6.4, 7.0, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-647
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pI Standard Ladder 4
pI Standard Ladder 4: 4.9, 5.5, 6.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-024
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pI Standard, 4.0
pI Standard, 4.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-025
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pI Standard, 4.2
pI Standard, 4.2 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-026
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pI Standard, 4.4
pI Standard, 4.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-027
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pI Standard, 4.9
pI Standard, 4.9 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-028
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pI Standard, 5.5
pI Standard, 5.5 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-029
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pI Standard, 6.0
pI Standard, 6.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-030
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pI Standard, 6.4
pI Standard, 6.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-031
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pI Standard, 7.0
pI Standard, 7.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-032
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pI Standard, 7.3
pI Standard, 7.3 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
041-036
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pI Standard, 8.4
pI Standard, 8.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-790
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pI Standard, 9.7
pI Standard, 9.7 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-967
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Premix G2, ampholyte-free separation gradient
Premix G2, ampholyte-free separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-968
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Premix G2, pH 3–10 separation gradient
Premix G2, pH 3–10 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-970
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Premix G2, pH 4–7 (nested) separation gradient
Premix G2, pH 4–7 (nested) separation gradient, pH 2-4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-969
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Premix G2, pH 4–9 separation gradient
Premix G2, pH 4–9 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-971
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Premix G2, pH 5–6 separation gradient
Premix G2, pH 5–6 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-972
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Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug
Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-973
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Premix G2, Pharmalyte pH 5–8 separation gradient
Premix G2, Pharmalyte pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-974
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Premix G2, Servalyt pH 5–8 separation gradient
Premix G2, Servalyt pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-337
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Anolyte Refill
Anolyte Refill (200 mL)
040-338
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Catholyte Refill
Catholyte Refill (200 mL)
041-108
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Wash Concentrate Refill for Peggy or NanoPro 1000
Wash Concentrate Refill (200 mL). REAGENT FOR PEGGY or NANOPRO 1000 USERS ONLY. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-652
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Luminol
Luminol (2 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-483
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RIPA Lysis Buffer
RIPA Lysis Buffer (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-764
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Bicine/CHAPS Lysis Buffer and Sample Diluent
Bicine/CHAPS Lysis Buffer and Sample Diluent (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-510
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DMSO Inhibitor Mix
DMSO Inhibitor Mix, 50X (200 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-482
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Aqueous Inhibitor Mix
Aqueous Inhibitor Mix, 25X (400 µL); Ships overnight on dry ice. Upon receipt, store at -20°C.
040-649
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Sample Diluent
Sample Diluent (3 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-309
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Antibody Diluent
Antibody Diluent (10 mL)
041-428
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DTT for Peggy or Sally
Single-use DTT - strip of 8 tubes. For Peggy or Sally.
041-595
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Instrument Cup (set of 3)
Includes set of 3 instrument cups
041-270
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Streptavidin-HRP for Simon, Sally, or Peggy
Streptavidin-HRP Conjugate (120 µl) (ready-to-use reagent) for use with Simon, Peggy and Sally
041-606
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Pipetting Guide
Pipetting guide for Sally, Peggy, Sally Sue or Peggy Sue Size Assay

Consumables

Part # Description
MR700
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Capillaries - Size Separation for Peggy/Sally (1 pack)
Peggy/Sally Capillaries for size assays (1 box of 96 capillaries)
MR701
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Capillaries - Size Separation for Peggy/Sally (5 pack)
Peggy/Sally Capillaries for size assays (5 boxes of 96 capillaries)
CBS700
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Capillaries-Charge Separation (1 pack) for Peggy or NanoPro 1000
Peggy, Peggy Sue and NanoPro 1000 Capillaries for charge assays (1 box of 96 capillaries)
CBS701
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Capillaries-Charge Separation (5 pack) for Peggy or NanoPro 1000
Peggy, Peggy Sue and NanoPro 1000 Capillaries for charge assays (5 boxes of 96 capillaries)
040-663
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Assay Plate/Lid Kit (5-Pack) for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000
Includes five 384-well plates and lids. * Must be ordered with p/n 041-528.
041-528
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Sponge Pack for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000
Includes 6 manifold cleaning sponges. * Must be ordered with p/n 040-663.