Peggy Sue reagents and consumables

Certified antibodies

Peggy Sue reagents and consumables are designed for use on with Peggy Sue and must be used in conjunction with Compass Software version 2.5 or higher and compatible firmware.

Size Assay Modules

Part # Description
DM-001
Buy Online
Anti-Rabbit Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes : Luminol-S, Peroxide, Antibody Diluent 2, Anti-Rabbit Secondary Antibody, Streptavidin-HRP.
DM-002
Buy Online
Anti-Mouse Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes : Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP.
DM-003
Buy Online
No secondary Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes : Luminol-S, Peroxide, Antibody Diluent 2, Streptavidin-HRP.
DM-004
Buy Online
Biotin Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes: Secondary Streptavidin HRP, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP (for biotinylated ladder).
DM-005
Buy Online
Anti-Human IgG Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes: Anti-Human IgG Secondary HRP Antibody, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP.
DM-006
Buy Online
Anti-Goat Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes: Anti-Goat Secondary HRP Antibody, Luminol-S, Peroxide, Milk-free Antibody Diluent (use to dilute goat primary antibody and as blocking agent), and Streptavidin-HRP. Upon receipt, store at 2-8°C.
DM-TP01
Buy Online
Total Protein Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes : Luminol-S, Peroxide, Antibody Diluent 2, Total Protein Reconstitution Agent 2, Total Protein Streptavidin-HRP.
SM-S001
Buy Online
12-230 kDa Peggy Sue or Sally Sue Separation Module
Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 2, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 2, Stacking Matrix 2, 10X Sample Buffer, Antibody Diluent (Store at RT), plus 2 x Standard Pack 1 clamshell with 8 ea. DTT, FL Standard master mix with 29 kDa System Control protein (Store at 4°C).
SM-S002
Buy Online
66-440 kDa Peggy Sue or Sally Sue Separation Module
Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 3, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 3, Stacking Matrix 2, 10X Sample Buffer, Antibody Diluent (Store at RT), plus 2 x Standard Pack 3 clamshell with 8 ea. DTT, FL Standard master mix with 90 kDa System Control protein (Store at 4°C).
SM-S003
Buy Online
2-40 kDa Peggy Sue or Sally Sue Separation Module
Requires Compass v3.0 or higher. Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 4, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 2, Stacking Matrix 2, 10X Sample Buffer, Antibody Diluent (Store at RT), plus 2 x Standard Pack 5 clamshell with 8 ea. DTT, FL Standard master mix with 26 kDa System Control protein (Store at 4°C).

Charge Assay Kits

Part # Description
CBS2001
Buy Online
XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000
Includes 5 boxes of capillaries, five 384-well plates with lids, Anolyte, Catholyte, Wash Concentrate, Luminol, and Peroxide XDR and manifold cleaning sponges.
CBS404
Buy Online
HeLa Lysate Controls Kit - Bicine/CHAPS Buffer for Charge Assays
Includes 5 vials each of low phospho and high phospho HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (5 µL/vial). Ships overnight on dry ice. Upon receipt, store components at 4°C.
CBS403
Buy Online
Lysis Kit - Bicine/CHAPS Buffer for Charge Assays
Includes Bicine/CHAPS Lysis Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix, and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C.
CBS401
Buy Online
Lysis Kit - RIPA Buffer for Charge Assays
Includes RIPA Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C.
041-126
Buy Online
Amplified Rabbit Secondary Antibody Detection Kit
Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Rabbit-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
041-127
Buy Online
Amplified Mouse Secondary Antibody Detection Kit
Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Mouse-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
PS-T003
Buy Online
Peggy System Charge Training Kit
Includes a XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 and additional reagents

Individual Reagents

Part # Description
PS-ST05-8
Buy Online
Standard Pack 5 2-40 kDa
Requires Compass v3.0 or higher. Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 2-40 kDa), Single Use FL Standard (26 kDa system control protein) and Single Use DTT vials. For use with Wes, Peggy Sue or Sally Sue Separation Modules.
PS-ST04-8
Buy Online
Standard Pack 4 66-440 kDa
Use with Compass v2.6 or higher. Includes 8 packets with one each of the Single Use Ladder 66-440 kDa, Single Use FL Standard 3 with 200 kDa system control protein and Single Use DTT vials. Reagents for 200 Simple Western data points. For use with Wes, Sally Sue, or Peggy Sue.
PS-ST03-8
Buy Online
Standard Pack 3 66-440 kDa
Use with Compass v2.6 or higher. Includes 8 packets with one each of the Single Use Ladder 66-440 kDa, Single Use FL Standard 3 with 90 kDa system control protein and Single Use DTT vials. Reagents for 200 Simple Western data points. For use with Wes, Sally Sue, or Peggy Sue.
PS-ST02-8
Buy Online
Standard Pack 2
Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa), Single Use FL Standard 2 (180 kDa system control protein) and Single Use DTT vials. Reagents for 200 Simple Western data points. For use with Wes, Sally Sue, or Peggy Sue.
PS-ST01-8
Buy Online
Standard Pack 1
Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa), Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. Reagents for 200 Simple Western data points. For use with Wes, Sally Sue, or Peggy Sue.
PS-FL01-8
Buy Online
Fluorescent 5x Master Mix 1
Includes 8 packets with one each of the Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. For use with Wes, Peggy Sue or Sally Sue 12-230 Separation Modules
PS-FL03-8
Buy Online
Fluorescent 5x Master Mix 3
Includes 8 packets with one each of the Single Use FL Standard (90 kDa system control protein) and Single Use DTT vials. For use with Wes, Peggy Sue or Sally Sue 66-440 kD Separation Modules
PS-FL05-8
Buy Online
Fluorescent 5x Master Mix 5
Includes 8 packets with one each of the Single Use FL Standard (26 kDa system control protein) and Single Use DTT vials. For use with Wes, Peggy Sue or Sally Sue 2-40 kD Separation Modules
043-226
Buy Online
Peggy Sue or Sally Sue 66-440 kDa Split Running Buffer Kit
Use with Compass v2.7 or higher. Includes both the Upper (043-209) and Lower (043-210) Running buffers 3.
043-225
Buy Online
Peggy Sue or Sally Sue 12-230 kDa Split Running Buffer Kit
Use with Compass v2.7 or higher. Includes both the Upper (043-163) and Lower (043-164) Running buffers 2.
043-326
Buy Online
Peggy Sue or Sally Sue 2-40 kDa Split Running Buffer 4 Kit
Requires Compass v3.0 or higher. Includes both the Upper (043-329) and Lower (043-330) Running buffers 4
042-976
Buy Online
Total Protein Streptavidin-HRP
Use with Compass v2.6 or higher. Streptavidin-HRP for Total Protein Assay for Wes, Sally Sue/Peggy Sue, 2.0 mL. Ready to use.
042-918
Buy Online
Peggy Sue/Sally Sue 66-440 kDa Separation Matrix
Use with Compass v2.6 or higher. Separation Matrix 3 for Peggy Sue or Sally Sue (2.5 mL)
043-328
Buy Online
Peggy Sue/Sally Sue 2-40 kDa Separation Matrix
Requires Compass v3.0 or higher. Separation Matrix 4 for Peggy Sue or Sally Sue (2.5 mL)
042-512
Buy Online
Separation Matrix II (2.5 mL) for Peggy Sue or Sally Sue
Separation matrix II (2.5 mL)
042-513
Buy Online
Stacking Matrix for Peggy Sue or Sally Sue
Stacking matrix II (1.5 mL)
042-195
Buy Online
Wes, Sally Sue, Peggy Sue Sample Buffer
Wes, Sally Sue, Peggy Sue 10X Sample Buffer (440 µL)
042-206
Buy Online
Anti-Rabbit Secondary Antibody
Goat Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue only.
042-205
Buy Online
Anti-Mouse Secondary Antibody
Goat Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue only.
043-426
Buy Online
20X Anti-Rabbit HRP Conjugate
20X Anti-Rabbit HRP Conjugate, 150 µL. To be diluted to 1X with RTU Anti-Mouse Secondary Antibody (042-205) when multiplexing with primary antibodies raised in mouse and rabbit.
042-203
Buy Online
Antibody Diluent 2
Antibody Diluent 2 for Wes, Peggy Sue and Sally Sue (20 mL). Ships overnight at room temperature. We strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Upon receipt, store at 4°C.
042-196
Buy Online
10X System Control Primary Antibody-Rabbit
To be used with rabbit primary antibody to Antibody Diluent. Detects system controls. Match with Standard pack 1, 2, 3 or 4 (250 µl/vial)
042-191
Buy Online
10X System Control Primary Antibody-Mouse
To be used with mouse primary antibody to Antibody Diluent. Detects system controls. Match with Standard pack 1, 2, 3 or 4 (250 µl/vial)
042-518
Buy Online
Matrix Removal Buffer for Peggy Sue or Sally Sue
Matrix Removal Buffer II (140 mL)
042-414
Buy Online
Streptavidin-HRP
Streptavidin-HRP Conjugate (ready-to-use reagent) for use with the Simple Western Size Standard.
042-520
Buy Online
Wash Buffer for Peggy Sue or Sally Sue
Wash Buffer (170 mL)
043-311
Buy Online
Luminol-S
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Luminol-S (2 mL)
043-379
Buy Online
Peroxide
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Peroxide (2 mL)
042-486
Buy Online
Erk 1 Primary Antibody for Size Assays
Erk 1 Rabbit Primary Antibody, 1X stock (1 mL)
042-488
Buy Online
HeLa Lysate Controls for Wes, Sally Sue and Peggy Sue
HeLa lysate, lysed in RIPA Lysis Buffer (20 µL/vial). For use with Wes, Sally Sue, or Peggy Sue.
043-522-2
Buy Online
Anti-Goat Secondary HRP Antibody
Donkey Anti-Goat Secondary HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Use our Milk-free Antibody Diluent (043-524) to dilute goat primary and as a blocking agent. Ships at room temperature. Upon receipt, store at 2-8°C.
043-491-2
Buy Online
Anti-Human IgG Secondary HRP Antibody
Goat Anti-Human Secondary HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Ships at room temperature. Upon receipt, store at 2-8°C.
043-459-2
Buy Online
Secondary Streptavidin-HRP
Secondary Streptavidin-HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Use with a biotinylated antibody. For total protein assay use 042-976 and to detect biotinylated molecular weight ladders use 042-414. Ships with ice pack. Upon receipt, store at 2-8°C.
043-524
Buy Online
Milk-free Antibody Diluent
Milk-free Antibody Diluent for Wes, Peggy Sue and Sally Sue (20 mL). We recommend its use for dilution of goat primary, anti-goat secondary and as blocking agent as it has been shown to be superior in reducing background compared to other diluents tested. Ships at room temperature. Upon receipt, store at 2-8°C.
040-655
Buy Online
Goat-Anti-Mouse-HRP Secondary Antibody
HRP-conjugated Goat-Anti-Mouse Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-656
Buy Online
Goat-Anti-Rabbit Secondary Antibody
HRP-conjugated Goat-Anti-Rabbit Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
041-081
Buy Online
Goat-Anti-Rabbit HUX HRP Secondary Antibody
HRP-Conjugated Goat-Anti-Rabbit Secondary Antibody cross-absorbed for minimal cross-reactivity against human antigens (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-474
Buy Online
Pan Erk Primary Antibody
Pan Erk Primary Antibody, 1X stock (1 mL)
040-477
Buy Online
Phospho-Erk 1/2 Primary Antibody
Phospho-Erk 1/2 Primary Antibody, 1X stock (1 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-644
Buy Online
pI Standard Ladder 1
pI Standard Ladder 1: 4.0, 4.9, 6.0, 6.4, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-645
Buy Online
pI Standard Ladder 2
pI Standard Ladder 2: 4.2, 4.9, 6.0, 6.4, 7.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-646
Buy Online
pI Standard Ladder 3
pI Standard Ladder 3: 4.9, 6.0, 6.4, 7.0, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-647
Buy Online
pI Standard Ladder 4
pI Standard Ladder 4: 4.9, 5.5, 6.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-024
Buy Online
pI Standard, 4.0
pI Standard, 4.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-025
Buy Online
pI Standard, 4.2
pI Standard, 4.2 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-026
Buy Online
pI Standard, 4.4
pI Standard, 4.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-027
Buy Online
pI Standard, 4.9
pI Standard, 4.9 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-028
Buy Online
pI Standard, 5.5
pI Standard, 5.5 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-029
Buy Online
pI Standard, 6.0
pI Standard, 6.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-030
Buy Online
pI Standard, 6.4
pI Standard, 6.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-031
Buy Online
pI Standard, 7.0
pI Standard, 7.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-032
Buy Online
pI Standard, 7.3
pI Standard, 7.3 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
041-036
Buy Online
pI Standard, 8.4
pI Standard, 8.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-790
Buy Online
pI Standard, 9.7
pI Standard, 9.7 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-967
Buy Online
Premix G2, ampholyte-free separation gradient
Premix G2, ampholyte-free separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-968
Buy Online
Premix G2, pH 3–10 separation gradient
Premix G2, pH 3–10 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-970
Buy Online
Premix G2, pH 4–7 (nested) separation gradient
Premix G2, pH 4–7 (nested) separation gradient, pH 2-4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-969
Buy Online
Premix G2, pH 4–9 separation gradient
Premix G2, pH 4–9 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-972
Buy Online
Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug
Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-973
Buy Online
Premix G2, Pharmalyte pH 5–8 separation gradient
Premix G2, Pharmalyte pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-974
Buy Online
Premix G2, Servalyt pH 5–8 separation gradient
Premix G2, Servalyt pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark.
040-337
Buy Online
Anolyte Refill
Anolyte Refill (200 mL)
040-338
Buy Online
Catholyte Refill
Catholyte Refill (200 mL)
041-108
Buy Online
Wash Concentrate Refill for Peggy or NanoPro 1000
Wash Concentrate Refill (200 mL). REAGENT FOR PEGGY or NANOPRO 1000 USERS ONLY. Ships overnight at room temperature. Upon receipt, store at 4°C.
040-652
Buy Online
Luminol
Luminol (2 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-483
Buy Online
RIPA Lysis Buffer
RIPA Lysis Buffer (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-764
Buy Online
Bicine/CHAPS Lysis Buffer and Sample Diluent
Bicine/CHAPS Lysis Buffer and Sample Diluent (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-510
Buy Online
DMSO Inhibitor Mix
DMSO Inhibitor Mix, 50X (200 µL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-482
Buy Online
Aqueous Inhibitor Mix
Aqueous Inhibitor Mix, 25X (400 µL); Ships overnight on dry ice. Upon receipt, store at -20°C.
040-649
Buy Online
Sample Diluent
Sample Diluent (3 mL); Ships overnight at room temperature. Upon receipt, store at 4°C.
040-309
Buy Online
Antibody Diluent
Antibody Diluent (10 mL)

Consumables

Part # Description
SS701
Buy Online
Capillaries - Size Separation for Peggy Sue/Sally Sue (5 pack)
Peggy/Sally and Peggy Sue/Sally Sue Capillaries for size assays (5 boxes of 96 capillaries)
SS700
Buy Online
Capillaries - Size Separation for Peggy Sue/Sally Sue (1 pack)
Peggy Sue/Sally Sue Capillaries for size assays (1 box of 96 capillaries)
031-102
Buy Online
Repeater Pipette for Peggy Sue or Sally Sue
Rainin AR-E1 positive-displacement electronic repeater pipette (0.1 - 50 mL). Use to dispense the Separation Matrix II for Sally Sue and Peggy Sue.
031-103
Buy Online
Repeater Pipette 0.5 mL Tips for Peggy Sue or Sally Sue
A box of 100 of the 0.5 mL syringe tips used with the Peggy Sue/Sally Sue Repeater Pipette.
MR700
Buy Online
Capillaries - Size Separation for Peggy/Sally (1 pack)
Peggy/Sally Capillaries for size assays (1 box of 96 capillaries)
MR701
Buy Online
Capillaries - Size Separation for Peggy/Sally (5 pack)
Peggy/Sally Capillaries for size assays (5 boxes of 96 capillaries)
040-663
Buy Online
Assay Plate/Lid Kit (5-Pack) for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000
Includes five 384-well plates and lids. * Must be ordered with p/n 041-528.
041-528
Buy Online
Sponge Pack for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000
Includes 6 manifold cleaning sponges. * Must be ordered with p/n 040-663.
041-606
Buy Online
Pipetting Guide
Pipetting guide for Sally, Peggy, Sally Sue or Peggy Sue Size Assay
041-595
Buy Online
Instrument Cup (set of 3)
Includes set of 3 instrument cups