Sally reagents and consumables

Sally reagents and consumables are designed for use with Sally and must be used in conjunction with Compass Software version 1.8 or higher and compatible firmware.

Assay Kits

Part # Description
CBS-01-01
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Rabbit (12-180 kDa) Size Separation Master Kit for Sally or Peggy
Core Kit for Peggy or Sally, 5 boxes of Capillaries (96 capillaries per box), Size Separation Kit for Peggy or Sally, Size Detection Kit-Rabbit for Peggy or Sally, and Simple Western Standard 1 (MW ladder spans 12-180 kDa). Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS-02-01
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Mouse (12-180 kDa) Size Separation Master Kit for Sally or Peggy
Core Kit for Peggy or Sally, 5 boxes of Capillaries (96 capillaries per box), Size Separation Kit for Sally or Peggy, Size Detection Kit-Mouse for Peggy or Sally, and Simple Western Standard 1 (MW ladder spans 12-180 kDa). Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS301
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Core Kit for Sally or Peggy
Includes Matrix Removal Buffer, Wash Buffer, Running Buffer, Luminol-S, Peroxide, Running Buffer cup, Wash Buffer cup, Matrix Removal cup, 5 Manifold Cleaning Sponges and 5 Plates. Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS301S1
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Size Separation Kit for Sally or Peggy
Includes Separation Matrix, Stacking Matrix, 2 Fluorescent Standard vials, Standard Resuspension Buffer, Sample Buffer and 8 DTT vials. Reagents for 480 Simple Western data points. Ships in 2 parts.
CBS301RD
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Size Detection Kit-Rabbit for Sally or Peggy
Goat-Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 480 Simple Western data points.
CBS301MD
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Size Detection Kit-Mouse for Sally or Peggy
Goat-Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 480 Simple Western data points.

Individual Reagents

Part # Description
CBS201STD1
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Simple Western Size Standard 1 for Sally, Peggy and Simon
Biotinylated Ladder 1 (MW ladder spans 12-180 kDa), Ladder Resuspension Buffer and Streptavidin-HRP. Reagents for 480 Simple Western data points.
041-695
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Single Use FL standard
Single Use FL standard-strip of 8 tubes (for use with Simple Western Size Standard 1 MW Ladder 12-180 kDa)
041-247
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Separation Matrix
Separation matrix 1 (2.5 mL)
041-248
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Stacking Matrix
Stacking matrix (1.5 mL)
041-265
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Simon, Sally, Peggy Sample Buffer
Simon, Sally, Peggy 10X Sample Buffer (400 µL)
041-808
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Antibody Diluent Plus
Antibody Diluent Plus for size assay (10 mL) for Simon, Sally or Peggy. Ships overnight at room temperature. Antibody Diluent is recommended for dilution of primary and secondary antibodies. Upon receipt, store at 4°C.
041-505
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Running Buffer for Sally or Peggy
Running Buffer (140 mL)
041-504
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Matrix Removal Buffer for Sally or Peggy
Matrix Removal Buffer (140 mL)
041-586
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Wash Buffer for Sally or Peggy
Wash Buffer (170 mL)
043-311
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Luminol-S
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Luminol-S (2 mL)
043-379
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Peroxide
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Peroxide (2 mL)
041-468
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HeLa Lysate Control - Bicine/CHAPS Buffer for Sally, Peggy or Simon
Includes 1 vial of HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (35 µL/vial)
041-270
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Streptavidin-HRP for Simon, Sally, or Peggy
Streptavidin-HRP Conjugate (120 µl) (ready-to-use reagent) for use with Simon, Peggy and Sally

Consumables

Part # Description
MR700
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Capillaries - Size Separation for Peggy/Sally (1 pack)
Peggy/Sally Capillaries for size assays (1 box of 96 capillaries)
MR701
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Capillaries - Size Separation for Peggy/Sally (5 pack)
Peggy/Sally Capillaries for size assays (5 boxes of 96 capillaries)
040-663
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Assay Plate/Lid Kit (5-Pack) for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000
Includes five 384-well plates and lids. * Must be ordered with p/n 041-528.
041-528
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Sponge Pack for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000
Includes 6 manifold cleaning sponges. * Must be ordered with p/n 040-663.
041-595
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Instrument Cup (set of 3)
Includes set of 3 instrument cups
041-606
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Pipetting Guide
Pipetting guide for Sally, Peggy, Sally Sue or Peggy Sue Size Assay
041-428
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DTT for Peggy or Sally
Single-use DTT - strip of 8 tubes. For Peggy or Sally.