Sally Sue reagents and consumables
Sally Sue reagents and consumables are designed for use on with Sally Sue and must be used in conjunction with Compass Software version 2.5 or higher and compatible firmware.
Assay Modules
| Part # | Description |
|---|---|
| DM-001 Buy Online |
Anti-Rabbit Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Rabbit Secondary Antibody, Streptavidin-HRP. |
| DM-002 Buy Online |
Anti-Mouse Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP. |
| DM-003 Buy Online |
No secondary Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Streptavidin-HRP. |
| DM-004 Buy Online |
Biotin Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Secondary Streptavidin HRP, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP (for biotinylated ladder). Compatible with Separation module - sold separately. |
| DM-005 Buy Online |
Anti-Human IgG Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Anti-Human IgG Secondary HRP Antibody, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP. Compatible with Separation module - sold separately. |
| DM-006 Buy Online |
Anti-Goat Detection Module Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Module includes: Anti-Goat Secondary HRP Antibody, Luminol-S, Peroxide, Milk-free Antibody Diluent (use to dilute goat primary antibody and as blocking agent), and Streptavidin-HRP. Compatible with Separation module - sold separately. |
| DM-TP01 Buy Online |
Total Protein Detection Module for Chemiluminescence Based Total Protein Assays Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. The Total protein detection module for Chemiluminescence based total protein assays includes Luminol-S, Peroxide, Antibody Diluent 2, Total Protein Reconstitution Agent 2, Total Protein Streptavidin-HRP. Compatible with Separation module - sold separately. |
| SM-S001 Buy Online |
12-230 kDa Peggy Sue or Sally Sue Separation Module Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 2, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 2, Stacking Matrix 2, 10X Sample Buffer, plus 2 x Standard Pack 1 clamshell with 8 ea. DTT, FL Standard master mix with 29 kDa System Control protein (Store at 4°C). |
| SM-S002 Buy Online |
66-440 kDa Peggy Sue or Sally Sue Separation Module Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 3, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 3, Stacking Matrix 2, 10X Sample Buffer, plus 2 x Standard Pack 3 clamshell with 8 ea. DTT, FL Standard master mix with 90 kDa System Control protein (Store at 4°C). |
| SM-S003 Buy Online |
2-40 kDa Peggy Sue or Sally Sue Separation Module Requires Compass v3.0 or higher. Use with a Detection Module. Reagents for 480 data points includes two parts: Separation box with 5 boxes of Capillaries (96 capillaries per box), 5 Plates, Wash Buffer, Upper and Lower Running Buffers 4, Upper and Lower Running Buffer cups, Wash Buffer cup, 5 Manifold Cleaning Sponges, Separation Matrix 2, Stacking Matrix 2, 10X Sample Buffer, plus 2 x Standard Pack 5 clamshell with 8 ea. DTT, FL Standard master mix with 26 kDa System Control protein (Store at 4°C). |
Individual Reagents
| Part # | Description |
|---|---|
| PS-ST05EZ-8 Buy Online |
EZ Standard Pack 5 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 2-40 kDa) and Single Use FL Standard (26 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST04EZ-8 Buy Online |
EZ Standard Pack 4 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 66-440 kDa) and Single Use FL Standard (200 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST03EZ-8 Buy Online |
EZ Standard Pack 3 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 66-440 kDa) and Single Use FL Standard (90 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST02EZ-8 Buy Online |
EZ Standard Pack 2 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa) and Single Use FL Standard (180 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-ST01EZ-8 Buy Online |
EZ Standard Pack 1 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa) and Single Use FL Standard (29 kDa system control protein) vials. Pack contains enough reagents to obtain 200 Simple Western data points. |
| PS-FL01-8 Buy Online |
Fluorescent 5x Master Mix 1 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. Compatible with 12-230 Separation Modules - sold separately. |
| PS-FL03-8 Buy Online |
Fluorescent 5x Master Mix 3 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (90 kDa system control protein) and Single Use DTT vials. Compatible with 66-440 kD Separation Modules - sold separately. |
| PS-FL05-8 Buy Online |
Fluorescent 5x Master Mix 5 Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (26 kDa system control protein) and Single Use DTT vials. Compatible with 2-40 kD Separation Modules - sold separately. |
| 043-226 Buy Online |
Peggy Sue or Sally Sue 66-440 kDa Split Running Buffer Kit Use with Compass v2.7 or higher. Includes both the Upper (043-209) and Lower (043-210) Running buffers 3. |
| 043-225 Buy Online |
Peggy Sue or Sally Sue 12-230 kDa Split Running Buffer Kit Use with Compass v2.7 or higher. Includes both the Upper (043-163) and Lower (043-164) Running buffers 2. |
| 043-326 Buy Online |
Peggy Sue or Sally Sue 2-40 kDa Split Running Buffer 4 Kit Requires Compass v3.0 or higher. Includes both the Upper (043-329) and Lower (043-330) Running buffers 4 |
| 042-976 Buy Online |
Total Protein Streptavidin-HRP Use with Compass v2.6 or higher. Streptavidin-HRP for Total Protein Assay Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems., 2.0 mL. Ready to use. |
| 042-918 Buy Online |
Peggy Sue/Sally Sue 66-440 kDa Separation Matrix Use with Compass v2.6 or higher. Separation Matrix 3 for Peggy Sue or Sally Sue (2.5 mL) |
| 043-328 Buy Online |
Peggy Sue/Sally Sue 2-40 kDa Separation Matrix Requires Compass v3.0 or higher. Separation Matrix 4 for Peggy Sue or Sally Sue (2.5 mL) |
| 042-512 Buy Online |
Separation Matrix II (2.5 mL) for Peggy Sue or Sally Sue Separation matrix II (2.5 mL) |
| 042-513 Buy Online |
Stacking Matrix for Peggy Sue or Sally Sue Stacking matrix II (1.5 mL) |
| 042-195 Buy Online |
Sample Buffer Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-206 Buy Online |
Anti-Rabbit Secondary Antibody Goat Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-205 Buy Online |
Anti-Mouse Secondary Antibody Goat Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-426 Buy Online |
20X Anti-Rabbit HRP Conjugate 20X Anti-Rabbit HRP Conjugate, 150 µL for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. To be diluted to 1X with RTU Anti-Mouse Secondary Antibody (042-205) when multiplexing with primary antibodies raised in mouse and rabbit. |
| 042-203 Buy Online |
Antibody Diluent 2 Antibody Diluent 2 (20 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. We strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. |
| 042-196 Buy Online |
10x System Control Primary Antibody (Rabbit) Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. To be used with rabbit primary antibody to Antibody Diluent. Detects system controls. Match with Standard pack 1, 2, 3 or 4 (250 µl/vial). |
| 042-191 Buy Online |
10x System Control Primary Antibody (Mouse) Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. The 10X System Control Primary Antibody-Mouse detects system controls is to be used with Antibody Diluent. *Please match accordingly with Standard packs 1, 2, 3 or 4 (250 µl/vial). |
| 042-518 Buy Online |
Matrix Removal Buffer for Peggy Sue or Sally Sue Matrix Removal Buffer II (140 mL) |
| 042-414 Buy Online |
Streptavidin-HRP Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. Streptavidin-HRP Conjugate (ready-to-use reagent) for use with the Simple Western Size Standard. |
| 042-520 Buy Online |
Wash Buffer for Peggy Sue or Sally Sue Wash Buffer (170 mL) |
| 043-311 Buy Online |
Luminol-S Luminol-S (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-379 Buy Online |
Peroxide Peroxide (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-486 Buy Online |
ERK 1 Primary Antibody for Size Assays Erk 1 Rabbit Primary Antibody, 1X stock (1 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 042-488 Buy Online |
HeLa Lysate Controls HeLa lysate, lysed in RIPA Lysis Buffer (20 µL/vial) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-522-2 Buy Online |
Anti-Goat Secondary HRP Antibody Donkey Anti-Goat Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. ProteinSimple recommends the use of our Milk-free Antibody Diluent (043-524) to dilute goat primary and as a blocking agent. |
| 043-491-2 Buy Online |
Anti-Human IgG Secondary HRP Antibody Goat Anti-Human Secondary HRP Conjugate (ready-to-use reagent) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-459-2 Buy Online |
Secondary Streptavidin-HRP Secondary Streptavidin-HRP Conjugate (ready-to-use reagent) for use with a biotinylated antibody in Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. For total protein assay use Secondary Streptavidin-HRP (P/N: 042-976) and to detect biotinylated molecular weight ladders use Streptavidin-HRP (P/N: 042-414). |
| 043-524 Buy Online |
Milk-free Antibody Diluent Milk-free Antibody Diluent compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. We recommend its use for dilution of goat primary, anti-goat secondary and as blocking agent as it has been shown to be superior in reducing background compared to other diluents tested. |
Consumables
| Part # | Description |
|---|---|
| SS701 Buy Online |
Capillaries - Size Separation for Peggy Sue/Sally Sue (5 pack) Peggy/Sally and Peggy Sue/Sally Sue Capillaries for size assays (5 boxes of 96 capillaries) |
| SS700 Buy Online |
Capillaries - Size Separation for Peggy Sue/Sally Sue (1 pack) Peggy Sue/Sally Sue Capillaries for size assays (1 box of 96 capillaries) |
| 031-102 Buy Online |
Repeater Pipette for Peggy Sue or Sally Sue Rainin AR-E1 positive-displacement electronic repeater pipette (0.1 - 50 mL). Use to dispense the Separation Matrix II for Sally Sue and Peggy Sue. |
| 031-103 Buy Online |
Repeater Pipette 0.5 mL Tips for Peggy Sue or Sally Sue A box of 100 of the 0.5 mL syringe tips used with the Peggy Sue/Sally Sue Repeater Pipette. |
| MR700 Buy Online |
Capillaries - Size Separation for Peggy/Sally (1 pack) Peggy/Sally Capillaries for size assays (1 box of 96 capillaries) |
| MR701 Buy Online |
Capillaries - Size Separation for Peggy/Sally (5 pack) Peggy/Sally Capillaries for size assays (5 boxes of 96 capillaries) |
| 040-663 Buy Online |
Assay Plate/Lid Kit (5-Pack) for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes five 384-well plates and lids. * Must be ordered with p/n 041-528. |
| 041-528 Buy Online |
Sponge Pack for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes 6 manifold cleaning sponges. * Must be ordered with p/n 040-663. |
| 041-606 Buy Online |
Pipetting Guide Pipetting guide for Sally, Peggy, Sally Sue or Peggy Sue Size Assay |
| 041-595 Buy Online |
Instrument Cup (set of 3) Includes set of 3 instrument cups |