Simon reagents and consumables

Simon reagents and consumables are designed for use on with Simon and must be used in conjunction with Compass Software version 1.8 or higher and compatible firmware.

Assay Kits

Part # Description
Simon-01-01
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Rabbit (12-180 kDa) Master Kit for Simon
Simon Core Kit, Simon Separation Kit 1, Simon Rabbit Detection Kit, and Simple Western Standard 1 (MW ladder spans 12-180 kDa). Reagents for 96 Simple Western data points. Ships in 2 parts.
Simon-02-01
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Mouse (12-180 kDa) Master Kit for Simon
Simon Core Kit, Simon Separation Kit 1, Simon Mouse Detection Kit, and Simple Western Size Standard 1 (MW ladder spans 12-180 kDa). Reagents for 96 Simple Western data points. Ships in 2 parts.
CBS201
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Core Kit for Simon
Matrix Removal Buffer, Wash Buffer, Running Buffer, Luminol-S, Peroxide, 1 box of 8 Capillary Clips (12 capillaries per clip), 2 Manifold Cleaning Sponges and 8 Plates. Reagents for 96 Simple Western data points. Ships in 2 parts.
CBS201S1
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Separation Kit 1 for Simon
Separation Matrix, Stacking Matrix, Fluorescent Standard, Standard Resuspension Buffer, Sample Buffer and 8 DTT vials. Reagents for 96 Simple Western data points. Ships in 2 parts.
CBS201RD
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Detection Kit-Rabbit for Simon
Goat-Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 96 Simple Western data points.
CBS201MD
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Detection Kit-Mouse for Simon
Goat-Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 96 Simple Western data points.

Individual Reagents

Part # Description
CBS201STD1
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Simple Western Size Standard 1 for Sally, Peggy and Simon
Biotinylated Ladder 1 (MW ladder spans 12-180 kDa), Ladder Resuspension Buffer and Streptavidin-HRP. Reagents for 480 Simple Western data points.
041-252
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Matrix Removal Buffer
Matrix Removal Buffer (20 mL)
041-249
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Running Buffer
Running buffer (15 mL)
041-427
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Wash Buffer
Wash Buffer (20 mL)
043-311
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Luminol-S
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Luminol-S (2 mL)
043-379
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Peroxide
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Peroxide (2 mL)
041-468
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HeLa Lysate Control - Bicine/CHAPS Buffer for Sally, Peggy or Simon
Includes 1 vial of HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (35 µL/vial)
041-695
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Single Use FL standard
Single Use FL standard-strip of 8 tubes (for use with Simple Western Size Standard 1 MW Ladder 12-180 kDa)
041-247
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Separation Matrix
Separation matrix 1 (2.5 mL)
041-248
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Stacking Matrix
Stacking matrix (1.5 mL)
041-270
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Streptavidin-HRP for Simon, Sally, or Peggy
Streptavidin-HRP Conjugate (120 µl) (ready-to-use reagent) for use with Simon, Peggy and Sally
041-808
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Antibody Diluent Plus
Antibody Diluent Plus for size assay (10 mL) for Simon, Sally or Peggy. Ships overnight at room temperature. Antibody Diluent is recommended for dilution of primary and secondary antibodies. Upon receipt, store at 4°C.
041-265
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Simon, Sally, Peggy Sample Buffer
Simon, Sally, Peggy 10X Sample Buffer (400 µL)

Consumables

Part # Description
MR CAPS
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Capillaries
Capillaries (8 clips)