Wes reagents and consumables

Certified antibodies

Wes reagents and consumables are designed for use with Wes and must be used in conjunction with Compass Software version 2.5 or higher and compatible firmware.

Assay Modules

Part # Description
DM-001
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Anti-Rabbit Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes : Luminol-S, Peroxide, Antibody Diluent 2, Anti-Rabbit Secondary Antibody, Streptavidin-HRP.
DM-002
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Anti-Mouse Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes : Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP.
DM-003
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No secondary Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes : Luminol-S, Peroxide, Antibody Diluent 2, Streptavidin-HRP.
DM-004
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Biotin Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes: Secondary Streptavidin HRP, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP (for biotinylated ladder).
DM-005
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Anti-Human IgG Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes: Anti-Human IgG Secondary HRP Antibody, Luminol-S, Peroxide, Antibody Diluent 2, and Streptavidin-HRP.
DM-006
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Anti-Goat Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes: Anti-Goat Secondary HRP Antibody, Luminol-S, Peroxide, Milk-free Antibody Diluent (use to dilute goat primary antibody and as blocking agent), and Streptavidin-HRP. Upon receipt, store at 2-8°C.
DM-TP01
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Total Protein Detection Module for Wes, Peggy Sue or Sally Sue
Use with a Wes or Sally Sue/Peggy Sue Separation Module. Includes : Luminol-S, Peroxide, Antibody Diluent 2, Total Protein Reconstitution Agent 2, Total Protein Streptavidin-HRP.
SM-W001
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12-230 kDa Wes Separation Module, 2 x 13 capillary cartridges
Use with a Detection Module. Reagents for 26 data points includes two parts: Separation box with 2 Capillary Cartridges (13 capillaries per cartridge), 2 Wes 12-230 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 1 clamshell with 2 ea. DTT, FL Standard master mix with 29 kDa System Control protein (Store at 4°C).
SM-W002
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12-230 kDa Wes Separation Module, 8 x 13 capillary cartridges
Use with a Detection Module. Reagents for 104 data points includes two parts: Separation box with 8 Capillary Cartridges (13 capillaries per cartridge), 8 Wes 12-230 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 1 clamshell with 8 ea. DTT, FL Standard master mix with 29 kDa System Control protein (Store at 4°C).
SM-W003
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12-230 kDa Wes Separation Module, 2 x 25 capillary cartridges
Use with a Detection Module. Reagents for 50 data points includes two parts: Separation box with 2 Capillary Cartridges (25 capillaries per cartridge), 2 Wes 12-230 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 1 clamshell with 2 ea. DTT, FL Standard master mix with 29 kDa System Control protein (Store at 4°C).
SM-W004
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12-230 kDa Wes Separation Module, 8 x 25 capillary cartridges
Use with a Detection Module. Reagents for 200 data points includes two parts: Separation box with 8 Capillary Cartridges (25 capillaries per cartridge), 8 Wes 12-230 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 1 clamshell with 8 ea. DTT, FL Standard master mix with 29 kDa System Control protein. (Store at 4°C).
SM-W005
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66-440 kDa Wes Separation Module, 2 x 13 capillary cartridges
Use with a Detection Module. Reagents for 26 data points includes two parts: Separation box with 2 Capillary Cartridges (13 capillaries per cartridge), 2 Wes 66-440 kDa Pre-filled Plates (pre-filled with Separation Matrix 3, Stacking Matrix 2, Split Running Buffer 3 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 3 clamshell with 2 ea. DTT, FL Standard master mix with 90 kDa System Control protein (Store at 4°C).
SM-W006
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66-440 kDa Wes Separation Module, 8 x 13 capillary cartridges
Use with a Detection Module. Reagents for 104 data points includes two parts: Separation box with 8 Capillary Cartridges (13 capillaries per cartridge), 8 Wes 66-440 kDa Pre-filled Plates (pre-filled with Separation Matrix 3, Stacking Matrix 2, Split Running Buffer 3 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 3 clamshell with 8 ea. DTT, FL Standard master mix with 90 kDa System Control protein (Store at 4°C).
SM-W007
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66-440 kDa Wes Separation Module, 2 x 25 capillary cartridges
Use with a Detection Module. Reagents for 50 data points includes two parts: Separation box with 2 Capillary Cartridges (25 capillaries per cartridge), 2 Wes 66-440 kDa Pre-filled Plates (pre-filled with Separation Matrix 3, Stacking Matrix 2, Split Running Buffer 3 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 3 clamshell with 2 ea. DTT, FL Standard master mix with 90 kDa System Control protein (Store at 4°C).
SM-W008
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66-440 kDa Wes Separation Module, 8 x 25 capillary cartridges
Use with a Detection Module. Reagents for 200 data points includes two parts: Separation box with 8 Capillary Cartridges (25 capillaries per cartridge), 8 Wes 66-440 kDa Pre-filled Plates (pre-filled with Separation Matrix 3, Stacking Matrix 2, Split Running Buffer 3 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 3 clamshell with 8 ea. DTT, FL Standard master mix with 90 kDa System Control protein (Store at 4°C).
SM-W009
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2-40 kDa Wes Separation Module, 2 x 13 capillary cartridges
Requires Compass v3.0 or higher. Use with a Detection Module. Reagents for 26 data points includes two parts: Separation box with 2 Capillary Cartridges (13 capillaries per cartridge), 2 Wes 2-40 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 4 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 5 clamshell with 2 ea. DTT, FL Standard master mix with 26 kDa System Control protein (Store at 4°C).
SM-W010
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2-40 kDa Wes Separation Module, 8 x 13 capillary cartridges
Requires Compass v3.0 or higher. Use with a Detection Module. Reagents for 104 data points includes two parts: Separation box with 8 Capillary Cartridges (13 capillaries per cartridge), 8 Wes 2-40 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 4 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 5 clamshell with 8 ea. DTT, FL Standard master mix with 26 kDa System Control protein (Store at 4°C).
SM-W011
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2-40 kDa Wes Separation Module, 2 x 25 capillary cartridges
Requires Compass v3.0 or higher. Use with a Detection Module. Reagents for 50 data points includes two parts: Separation box with 2 Capillary Cartridges (13 capillaries per cartridge), 2 Wes 2-40 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 4 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 5 clamshell with 2 ea. DTT, FL Standard master mix with 26 kDa System Control protein (Store at 4°C).
SM-W012
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2-40 kDa Wes Separation Module, 8 x 25 capillary cartridges
Requires Compass v3.0 or higher. Use with a Detection Module. Reagents for 200 data points includes two parts: Separation box with 8 Capillary Cartridges (25 capillaries per cartridge), 8 Wes 2-40 kDa Pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 4 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus Standard Pack 5 clamshell with 8 ea. DTT, FL Standard master mix with 26 kDa System Control protein (Store at 4°C).

Individual Reagents

Part # Description
PS-ST05-8
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Standard Pack 5 2-40 kDa
Requires Compass v3.0 or higher. Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 2-40 kDa), Single Use FL Standard (26 kDa system control protein) and Single Use DTT vials. For use with Wes, Peggy Sue or Sally Sue Separation Modules.
PS-ST04-8
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Standard Pack 4 66-440 kDa
Use with Compass v2.6 or higher. Includes 8 packets with one each of the Single Use Ladder 66-440 kDa, Single Use FL Standard 3 with 200 kDa system control protein and Single Use DTT vials. Reagents for 200 Simple Western data points. For use with Wes, Sally Sue, or Peggy Sue.
PS-ST03-8
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Standard Pack 3 66-440 kDa
Use with Compass v2.6 or higher. Includes 8 packets with one each of the Single Use Ladder 66-440 kDa, Single Use FL Standard 3 with 90 kDa system control protein and Single Use DTT vials. Reagents for 200 Simple Western data points. For use with Wes, Sally Sue, or Peggy Sue.
PS-ST02-8
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Standard Pack 2
Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa), Single Use FL Standard 2 (180 kDa system control protein) and Single Use DTT vials. Reagents for 200 Simple Western data points. For use with Wes, Sally Sue, or Peggy Sue.
PS-ST01-8
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Standard Pack 1
Includes 8 packets with one each of the Single Use Biotinylated Ladder (MW ladder spans 12-230 kDa), Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. Reagents for 200 Simple Western data points. For use with Wes, Sally Sue, or Peggy Sue.
PS-FL01-8
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Fluorescent 5x Master Mix 1
Includes 8 packets with one each of the Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. For use with Wes, Peggy Sue or Sally Sue 12-230 Separation Modules
PS-FL03-8
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Fluorescent 5x Master Mix 3
Includes 8 packets with one each of the Single Use FL Standard (90 kDa system control protein) and Single Use DTT vials. For use with Wes, Peggy Sue or Sally Sue 66-440 kD Separation Modules
PS-FL05-8
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Fluorescent 5x Master Mix 5
Includes 8 packets with one each of the Single Use FL Standard (26 kDa system control protein) and Single Use DTT vials. For use with Wes, Peggy Sue or Sally Sue 2-40 kD Separation Modules
042-976
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Total Protein Streptavidin-HRP
Use with Compass v2.6 or higher. Streptavidin-HRP for Total Protein Assay for Wes, Sally Sue/Peggy Sue, 2.0 mL. Ready to use.
042-414
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Streptavidin-HRP
Streptavidin-HRP Conjugate (ready-to-use reagent) for use with the Simple Western Size Standard.
042-195
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Wes, Sally Sue, Peggy Sue Sample Buffer
Wes, Sally Sue, Peggy Sue 10X Sample Buffer (440 µL)
042-206
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Anti-Rabbit Secondary Antibody
Goat Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue only.
042-205
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Anti-Mouse Secondary Antibody
Goat Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue only.
043-426
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20X Anti-Rabbit HRP Conjugate
20X Anti-Rabbit HRP Conjugate, 150 µL. To be diluted to 1X with RTU Anti-Mouse Secondary Antibody (042-205) when multiplexing with primary antibodies raised in mouse and rabbit.
042-203
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Antibody Diluent 2
Antibody Diluent 2 for Wes, Peggy Sue and Sally Sue (20 mL). Ships overnight at room temperature. We strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Upon receipt, store at 4°C.
042-196
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10X System Control Primary Antibody-Rabbit
To be used with rabbit primary antibody to Antibody Diluent. Detects system controls. Match with Standard pack 1, 2, 3 or 4 (250 µl/vial)
042-191
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10X System Control Primary Antibody-Mouse
To be used with mouse primary antibody to Antibody Diluent. Detects system controls. Match with Standard pack 1, 2, 3 or 4 (250 µl/vial)
042-202
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Wash Buffer
Wash Buffer (60 mL)
PS-CS01
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Chemiluminescent Substrate
Chemiluminescent Substrate (2 bottles, 2.0 mL/bottle). Includes Luminol-S (042-233) and Peroxide (042-234).
042-486
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Erk 1 Primary Antibody for Size Assays
Erk 1 Rabbit Primary Antibody, 1X stock (1 mL)
042-488
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HeLa Lysate Controls for Wes, Sally Sue and Peggy Sue
HeLa lysate, lysed in RIPA Lysis Buffer (20 µL/vial). For use with Wes, Sally Sue, or Peggy Sue.
043-311
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Luminol-S
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Luminol-S (2 mL)
043-379
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Peroxide
Wes, Peggy Sue, Sally Sue, Peggy, Sally or Simon Peroxide (2 mL)
043-522-2
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Anti-Goat Secondary HRP Antibody
Donkey Anti-Goat Secondary HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Use our Milk-free Antibody Diluent (043-524) to dilute goat primary and as a blocking agent. Ships at room temperature. Upon receipt, store at 2-8°C.
043-491-2
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Anti-Human IgG Secondary HRP Antibody
Goat Anti-Human Secondary HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Ships at room temperature. Upon receipt, store at 2-8°C.
043-459-2
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Secondary Streptavidin-HRP
Secondary Streptavidin-HRP Conjugate (ready-to-use reagent). For Wes, Sally Sue, and Peggy Sue (2 x 1 mL). Use with a biotinylated antibody. For total protein assay use 042-976 and to detect biotinylated molecular weight ladders use 042-414. Ships with ice pack. Upon receipt, store at 2-8°C.
043-524
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Milk-free Antibody Diluent
Milk-free Antibody Diluent for Wes, Peggy Sue and Sally Sue (20 mL). We recommend its use for dilution of goat primary, anti-goat secondary and as blocking agent as it has been shown to be superior in reducing background compared to other diluents tested. Ships at room temperature. Upon receipt, store at 2-8°C.

Consumables

Part # Description
PS-PP05
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Wes 2-40 kDa Pre-filled Plates
Requires Compass v3.0 or higher. 8 Plates (pre-filled with Separation Matrix, Stacking Matrix, Split Running Buffer and Matrix Removal Buffer).
PS-PP04
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Wes 66-440 kDa Pre-filled Plates with Split Buffer
Use with Compass v2.7 or higher. 8 Plates (pre-filled with Separation Matrix, Stacking Matrix, Split Running Buffer 3 and Matrix Removal Buffer).
PS-PP03
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Wes 12-230 kDa Pre-filled Plates with Split Buffer
Use with Compass v2.7 or higher. 8 Plates (pre-filled with Separation Matrix, Stacking Matrix, Split Running Buffer 2 and Matrix Removal Buffer).
PS-PP02
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Wes 66-440 kDa Pre-filled Plates
Use with Compass v2.6 or higher. 8 Plates (pre-filled with Separation Matrix 3, Stacking Matrix 2, Running Buffer 3 and Matrix Removal Buffer).
PS-PP01
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Wes Pre-filled Plates
8 Plates (pre-filled with Separation Matrix, Stacking Matrix, Running Buffer and Matrix Removal Buffer).
PS-CC01
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Wes 25-Capillary Cartridges (8 pack)
Wes Capillaries (8 cartridges - 25 capillaries/cartridge)
PS-CC02
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Wes 13-Capillary Cartridges (8 pack)
Wes Capillaries (8 cartridges - 13 capillaries/cartridge)