Ella

Unprecedented Reproducibility

Drug development researchers are constantly challenged by the reproducibility and variability of an immunoassay. This is especially difficult when transferring an assay from one department to another or from one department to a contract research organization. Ella addresses this challenge head on by utilizing microfluidic cartridges that include pre-loaded reagents so only the samples and wash buffer are loaded onto the cartridge. From there, Ella takes over and after one hour, the concentration of the analyte is automatically calculated. Have confidence in our continuously expanding menu of Simple Plex™ assays, powered by R&D Systems antibodies and buffers and validated against our gold standard Quantikine® ELISAs.

Ella

Single or multi-analyte — you pick

Simple Plex assays are provided as single analyte assays with 72x1 cartridge and multiplex assays with 16x4 cartridge. That means you can analyze 72 samples for a single analyte or 16 samples for four analytes, giving you the flexibility your assay needs. Standard curves are calculated at the factory and downloaded when the cartridge barcode is read—there is no need to make standard dilutions improving reproducibility and accuracy.

All the assays you need

Powered by R&D Systems, our Simple Plex assays are correlated to our Quantikine® ELISAs components so quality is a slam-dunk. Our large and continuously growing menu of assays has been thoroughly validated to ensure you get the best performance. Don't see something you need on the list? Just let us know and we'll make something specific just for you!

Faster Workflow

Setup with Ella only takes 10-15 minutes, and only needs 25 µL of sample. Just scan the barcode, add your samples and wash buffer to the microfluidics cartridge, load the cartridge and press start—Ella automates every step. So all that hands-on, manual washing and reagent adding you'd do with a typical ELISA? Gone. You'll have results in an hour, and Ella does all the data analysis for you too.

Traditional ELISA
80-120 min setup
3-6 hr for results

Ella
10-15 min setup
1 hr for results

Technology & Applications

Microfluidics and nanoreactors improves accuracy

Simple Plex assays are a lot like ELISAs, just without the errors that come with multiple, manual steps. Integrating microfluidics and three nanoreactors per reaction ensures accuracy and precision—there is no need to run technical replicates per sample. Ella uses fluorescence detection to up your sensitivity, and gives you the specificity you'd expect from your ELISA. That all adds up to better CVs, a broader dynamic range, and the ability to run low and high level samples in one assay!

Multiplex without cross-reactivity

There's no cross-reactivity with Simple Plex assays. Ella splits your sample across four distinct channels when you multiplex on the 16 x 4 cartridge. So it's just like running four separate ELISAs at once. End result? Way better accuracy and broader dynamic range than you can ever get with multiplexed ELISA.

Figure 1.  Comparison of the dynamic range and sensitivity of a Simple Plex multi-analyte assay (IL-1β, IL-5, IL-10 and IL-12 in a single panel) run on Ella with those from commercial single-plex ELISAs for the same analytes. You'll get comparable results with single analyte and multi-analyte assays.

Easily detect low endogenous levels

Figure 2.  Simple Plex assays have a broader dynamic range and lower detection limit than standard manual ELISAs as shown.

Get reproducible results without worrying about pipetting technique

Figure 3.  Reproducibility for 4 separate analytes in a Simple Plex assay. Plots show calculated concentration (pg/mL) at various volumes loaded into the cartridge. The observed concentration had exceptional CVs at all sample loads. So you don't need great pipetting technique to get excellent results, the assay format is very forgiving.

Built-in standard curves and triplicate results saves lots of time

Calibration

Figure 4.  Simple Plex cartridge factory calibration compared to daily in-lab calibration. When you scan the cartridge barcode, it gives Ella the factory-calibrated standard curve for your assays. You don't have to prepare a standard curve which also improved precision of the assay.

User to user and site to site reproducibility

Site to site reproducibility

Figure 5.  Reproducibility of the Simple Plex platform was tested across 3 sites, 11 users and 9 Ella instruments. 4 different assays were processed for CCL2, IL-6, TNFalpha and VEGF-A. 8 unique serum samples with 2 controls for a total of 704 results.

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