FluorChem E/M Application Data
- Ultrasensitive Chemiluminescent Imaging
- Quantitative Fluorescent Detection
- Multiplex Fluorescence
Ultrasensitive Chemiluminescent Imaging
Never miss a faint band again! Digital imaging on the FluorChem E and M captures a dynamic range that surpasses film in a shorter time. Get a superior signal while loading less protein and using lower antibody titers.
Quantitative Fluorescent Detection
Direct fluorescent detection is a stable, non-enzymatic alternative to chemiluminescent imaging. Achieve greater linearity over a broader dynamic range on the FluorChem M, allowing for more accurate quantitation. MultiFluor Western Blotting kits are optimized for performance and include everything you need for secondary fluorescence detection.
Serial dilution of Transferrin (1.0 µg to 0.5 pg) detected by Western blot using MultiFluor Green secondary antibody. The resulting linear range was greater than three orders of magnitude with 0.5 pg limit of detection.
Fluorescent Western blot detection of a Transferrin dilution series using MultiFluor Green secondary antibody yielded a dynamic range of approximately five logs.
Multiplex Fluorescence
Probe with up to three fluorescent secondary antibodies in the same blot. No more protein loss and time wasted with stripping and re-probing. Band intensity values can also be normalized to a loading control to correct for inaccuracies.
HeLa lysates at 4 µg, 1 µg and 0.25 µg probed with anti-Hsp-70, anti-β-Actin and anti-ERK 1/2 primary antibodies followed by MultiFluor Green, MultiFluor Blue, and MultiFluor Red secondary antibodies.
Proteins that overlap due to similar migrating patterns can also be probed on the same blot. The resulting image data for each protein can be analyzed independently or overlaid to readily detect subtle mobility shifts and identify co-migrating bands.
HeLa lysates probed with anti-ERK 1/2 and anti-pERK 1/2 primary antibodies followed by MultiFluor Green and MultiFluor Red secondary antibodies.