FluorChem Q system

Quantitative Western blot imaging

FluorChem Q

The multiplex fluorescent detection and chemiluminescent imaging capabilities of the FluorChem™ Q system is a slam-dunk solution for quantitative Western blot imaging and analysis. Its open platform is compatible with a wide range of dyes and kits, so you'll have full flexibility when you need it for multiple applications.

  • Six-position filter wheel gives you full-on UV and fluorescent imaging versatility
  • Three excitation channels with specific wavelengths let you do multiplex fluorescent Western blot imaging
  • Powerful fixed lens for imaging speeds that fly past film every time
  • AlphaView® Q software saves imaging protocols and has all the quantitative analysis tools you need

Technology & Applications

Look at multiple proteins simultaneously

The FluorChem Q system lets you do quantitative detection of multiple proteins in a single assay, and there's no need to strip and re-probe blots. Intensity values are easily normalized to a loading control to correct for any inaccuracies.

Cy3 Labeled CEA Experimental Protein 1

Cy5 Labeled transferrin Experimental Protein 2

Fluorescein Labeled IgG Loading Control

Composite image of a three-color fluorescent Western blot, carcino embryonic antigen (CEA), transferrin and IgG were detected with fluorescently labeled antibodies, and imaged in three spectrally unique fluorescent channels. Imaging time: 24 seconds.

See co-migrating proteins

Overlapping proteins like phosphorylated and unphosphorylated isoforms or other proteins with similar migration can be probed in the same blot. And our unique Channel Viewer feature lets you visualize individual bands in overlapping areas.

Ultra-sensitive protein detection

The FluorChem Q system gives you the wide dynamic range and sensitivity you need to accurately detect even the most subtle changes in protein levels.

Serial dilutions of transferrin detected by Western blot with a secondary Cy5 labeled antibody and imaged on the FluorChem Q system. A lower limit of 0.6 pg transferrin was detected.