iCE280 System Overview

iCE280 system

The iCE280 System performs free solution IEF in a capillary column (cIEF) and detects focused protein zones using a whole column UV absorption detector that avoids disturbing these focused zones. This technology is unique in that it has the comparable resolution of traditional gel IEF but incorporates the advantages of a column based separation technology, including quantitation and automation.

It is superior to conventional cIEF performed with single point detection systems, which require a lengthy mobilization phase. As a result, method development and sample analysis can be run on the iCE280 in a fraction of the time required by other technologies and our resolution and reproducibility are superior.

The iCE280, with whole-column detection, completely eliminates the need for a mobilization phase. This results in a unique combination of speed, resolution and reproducibility.

Product Specifications

  • Separation Technique: Free solution whole column detection capillary isoelectric focusing (cIEF)
  • Separation Column: 100 µm ID x 50 mm (2") long fluorocarbon coated capillary
  • Detection Method: Whole column light absorption at 280 nm
  • Linear Detection Range at 280 nm: > 100
  • Focusing Voltage: Constant voltage, stepwise adjustable from 100 to 600 volts/cm
  • Automation: Choice of PrinCE MicroInjector or Alcott 719AL Autosampler
  • Sample Throughput: Up to 7 injections per hour
  • Ambient Temperature: 18°C to 23°C
  • Relative Humidity: <= 85% non-condensing
  • Electrical: 115/220 VAC +/- 10%, 50 – 60 Hz +/- 0.5%
  • Size: 28.25 cm wide x 60.5 cm high x 31 cm deep (11.125" W x 23.75" H x 12.25" D)
    (Note: The iCE280 sits on a stand (14.6 cm / 6.625" high) when used with the PrinCE MicroInjector)
  • Weight: 45 pounds (20 kg)

iCE280 Options

AutoSamplers

Part # Description
PrinCE MicroInjector PrinCE MicroInjector
The PrinCE MicroInjector is the preferred option in an environment where walk-up robustness is critical. It provides good sample throughput and requires a smaller sample volume.
  • Throughput: up to 4 - 5 injections/hour
  • Tray capacity: 40 @ 11mm vials - not microtiter plate compatible
  • Maximum injections (CFR v2.3): up to 160 in up to 60 hours
  • Typical sample volume: 10 µL
  • Refrigeration: 8°C to 40°C
  • Electrical: 115/230 VAC +/- 10%, 50 – 60 Hz
  • Size: 34.5 cm (13 5/8") Wide x 46 cm (18") High x 49 cm (19 1/2") Deep
  • Weight: 77 pounds (35 kg) with packaging, 62 pounds (28 kg) without packaging
Alcott Autosampler 719A Alcott Autosampler 719A
The Alcott Autosampler is preferred when the iCE280 system is used frequently for a large number of injections - run up to 200 samples in up to 60 hours. For even greater convenience, add the Maximizer option with continuous flow pump and 96 well microtiter plate format.
  • Throughput: up to 7 injections/hour
  • Tray capacity: 50 @ 15mm vials or 96 well microtiter plate
  • Maximum injections (CFR v2.3): up to 200 in up to 60 hours
  • Typical sample volume: 40 µL
  • Refrigeration: 8°C to 40°C
  • Electrical: 115/230 VAC +/- 10%, 50 – 60 Hz
  • Size: 32.5 cm (12 3/4") Wide x 33.5 cm (13 1/8") High x 55 cm (21.5") Deep
  • Weight: 48 pounds (22 kg) with packaging, 35 pounds (16 kg) without packaging

Maximizer

Part # Description
iCE280 IEF Analyzer System with Alcott Analyzer iCE280 IEF Analyzer System with Alcott Analyzer
Welcome to the world of high throughput!
  • Increase productivity
  • Enhance flexibility
The Maximizer High Throughput option includes a continuous flow pump and microtiter plate adapter.

Choose a 96 well microtiter plate (single use or reusable) from any manufacturer. Use a robotic system to fully automate sample preparation.

No extra footprint is required as the continuous flow pump is located on a stand (provided by Convergent) above the Alcott.