Maurice

Your workhorse station for purity, identity and heterogeneity analysis

Maurice innovates the conventional capillary electrophoresis technology to automate your protein profiling either by size or charge. Need cIEF and CE-SDS data for your mAbs, ADCs, vaccines or virus-like particles? Maurice does it all. Just pop in one of the preassembled cartridges, drop in your sample vials or a 96-well plate, and hit start. You will complete method development in a day; and get high-resolution, reproducible data in minutes.

Maurice

How Can Maurice Help You?

My molecule has complicated charge profiles that are difficult to resolve

Maurice affords high-resolution characterization of charge variants by preserving our proprietary imaged capillary isoelectric focusing (icIEF) technology, which has been the go-to method for measuring charge heterogeneity. The whole-column detection eliminates the post-focusing mobilization step needed with traditional cIEF, and can resolve your most challenging molecules including fusion proteins and hydrophobic ADCs.

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My current method is not reproducible and error-prone

Maurice employs a prequalified, ready-to-use cartridge design which fully automates column conditioning and clean up. No laborious capillary assembly or maintenance is required. The on-board sample mixing feature further enhances the ease-of-use, and reduces operator-dependent variability commonly encountered with other systems.

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I have many samples and my schedule is tight

Maurice produces pI and charge heterogeneity data in less than 10 minutes, and size-based CE-SDS data in 35 minutes. The fast separation time, flexible workflow and high reproducibility makes Maurice a true lab workhorse. In one day you can complete your method development, or analyze 48 to 100 samples in one batch. The icing? You can develop platform methods and use them for multiple molecules too.

I am concerned about cross-contamination between applications

Maurice employs completely separate fluid paths for cIEF and CE-SDS applications. Waste is collected inside the cartridge which removes the concern about cross-contamination between experiments. In addition, when dealing with molecules such as ADCs, no more hassle about handling the toxic waste.

My sample is limited and they tend to aggregate at high concentration

Maurice's native fluorescence detection for cIEF provides 3-5X higher sensitivity than UV absorption, which means you can now measure sample at concentration as low as 0.7 µg/ml. And because proteins tend to aggregate less at lower concentrations you can reduce or even eliminate urea completely in some of your methods. Baselines are also significantly cleaner and less sensitive to ampholyte interference, giving you more options when optimizing your pH gradient.

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I work in a GMP lab and data integrity is key to my compliance requirement

Compass for iCE, the software package Maurice uses to acquire, manage and analyze data, has all the tools required for 21 CFR compliance so you'll have no issue analyzing biotherapeutics on Maurice in a regulated environment.

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Specifications
FEATURE cIEF SPECIFICATION CE-SDS SPECIFICATION
Minimum Sample Volume 50 µL 50 µL
Sample Delivery Vacuum Electrokinetic
Typical Separation Time 6–10 min (molecule-dependent) Reduced IgG: 25 min,
Non-reduced IgG: 35 min
Detection Capability UV Absorbance at 280 nm
Fluorescence: Ex 280 nm, Em 320–450 nm
UV Absorbance at 220 nm
Typical Voltage Pre-focusing: 1500 V, focusing: 3000 V Separation: 5750 V
Sample Injections per Cartridge 100 guaranteed, 200 maximum 100 guaranteed, 200 maximum
Maximum Sample Injections per Batch 100 48
pI/Size Range 2.85–10.45 10–240 kDa
pI/Sizing CV 1% ≤2%
CV for Peaks >10% Composition ≤5% (Intra-batch), ≤6% (Inter-batch) N/A
Relative Migration Time CV N/A <1% for reduced IgG
pI/Sizing Resolution 0.05 pI units (for wide range 3–10 ampholytes) ≥1.5 for NGHC/HC IgG Standard
Dynamic Range 2 logs 2 logs
Linearity >0.995 >0.995
Sensitivity (LOD) 0.7 µg/mL (Native fluorescence)
3.0 µg/mL (Absorbance)
(Values based on a monoclonal antibody)
0.3 µg/mL
(Value based on Internal Standard)
Sample Tray Options 96-well plates or 48 vials 96-well plates or 48 vials