Maurice C.
Identity and charge heterogeneity
Maurice C. runs cIEF applications on your mAbs, ADCs, and vaccines faster than anything out there. We're talking 10 minutes per sample and method development in a day. You get reliable and reproducible data day in and day out with single-digit CVs, and resolution that trumps HPLC.
He runs cIEF application on your mAbs, ADCs, and vaccines faster than anything out there. We're talking 10 minutes per sample and method development in a day. He'll even give you data on their variants. Already use an iCE system? Method transfer to Maurice is a breeze!
Technology & Applications
cIEF on Maurice C.
Figure 1. Just add anolyte and catholyte to a cIEF cartridge and pop it in Maurice. Then just drop in your sample vials or a 96-well plate, and hit start—he does the rest!
Figure 2. Charge profile of mAb. 0.25 mg/mL mAb prepared with 4% 3-10 Pharmalyte, 10 mM arginine, iminodiacteic acid (IDA) and 2 M urea, pI markers 4.05 and 9.99. Pre-focused at 1500 V for 1 min followed by focusing for 7 mins at 3000 V.
More iCE
Maurice uses whole column imaging technology for his cIEF methods. Translation? You get crazy reliable and reproducible data day in and day out with single-digit CVs, and resolution that trumps HPLC. Here's where he really ups the ante: he gives you absorbance and native fluorescence data on every sample, so you can analyze unmodified molecules with sensitivity down to 0.7 µg/mL!
Figure 3. (A) cIEF application performance over 100 injections with peak area CVs consistently less than 4%. 0.25 mg/mL mAb prepared with 4% 3-10 Pharmalyte, 10 mM arginine and iminodiacteic acid, pI markers 4.05 and 9.99. (B) Six overlaid injections of peptide markers at 0.0015-0.003 mg/mL,4% 3-10 Pharmalyte, 10 mM arginine and iminodiacteic acid, with flanking pI markers 3.38 and 10.17.