Need protein validation of your Single-cell RNA sequencing (RNA-Seq) data?
Do you have a list of targets from your Single-cell RNA sequencing (RNA-Seq) experiments and want to verify protein expression levels for your targets? Only Single-Cell Westerns offer the versatility to validate diverse protein targets discovered in your sequencing runs.
Milo provides protein heterogeneity information to validate your single-cell RNA data. Since mRNA levels do not always correlate with functional protein levels, pairing single-cell RNA data with single-cell protein expression data is critical to making accurate and complete conclusions about cellular function. Because Milo uses the large Western catalog of antibodies & can easily measure proteins irrespective of their location in or on a cell, Milo is the only platform with the versatility to detect diverse targets that are discovered in your sequencing runs.
How does Milo integrate into my Single-cell RNA sequencing (RNA-Seq) workflow?
You can split your sample and run half through a Single-cell RNA sequencing (RNA-Seq) workflow and half through the Milo Single-Cell Western workflow. Before probing your scWest chips, you can archive the chips until you have the results of your sequencing results – up to 9 months! Then select antibodies from the large commercial catalog of Western-validated antibodies to probe the scWest chips for your targets of interest. When you’re done, you will have single-cell protein heterogeneity data to validate your single-cell RNA data on the same sample.
Download our application note to learn how Milo was used in parallel with a single-cell RNA-Seq workflow at the Stanford Functional Genomics Facility to validate single-cell RNA expression studies with single-cell protein expression data and ensure accurate and complete conclusions about cellular function.
Want to learn more about Milo?
Watch the video and see how Milo can automate 1,000 single cell separations in just 4 hours.