NanoPro 1000

Simple Western charge assays

NanoPro 1000

Characterizing proteins is the specialty of NanoPro™ 1000. As the first Simple Western system for charge-based assays, NanoPro 1000 was designed to give you very detailed information about your proteins—including characterization of post-translational protein modifications.

Technology & Applications

Simple Western charge assays on the NanoPro 1000

Now, you can understand your proteins in detail. Is it 1 isoform or 7? Is it phosphorylated, acetylated, or methylated? The NanoPro 1000 will show just what's going on with your proteins.

NanoPro ERK data

Figure 1.  Using a pan-ERK primary antibody, the NanoPro 1000 separates non-phosphorylated ERK isoforms from mono and di-phosphorylated isoforms.

Fine needle aspirates (FNAs) taken from mouse xenografts typically contain too few cells for traditional protein analysis. But, these samples have plenty of material for Simple Western charge-based assay analysis! In fact, investigators from Stanford University have used FNA analysis on the NanoPro 1000 to monitor drug effects in individual mice over time.

Figure 2.  Gleevec decreases STAT5 phosphorylation in mouse xenografts of leukemia cells. The data shown was generated from a tumor xenograft formed following subcutaneous injection of leukemia cells into a SCID mouse. The Gleevec-induced decrease in STAT5 phosphorylation was dramatic, while the level of HSP70 loading control remained essentially constant.

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