Simple Western systems comparison chart

Which system is right for you?

Simple Western System Wes Sally Sue Peggy Sue NanoPro 1000
Simple Western size assays  
Simple Western charge assays    
Maximum samples per run 25 96 96 96
Run time for max samples (size) < 3 hours 14-19 hours 11-19 hours N/A
Run time for max samples (charge) N/A N/A 11 hours 11 hours
Sample cooling (size) N/A 10°C 10°C N/A
Sample cooling (charge) N/A N/A 3°C 3°C
Operating humidity range 20-60% relative, non-condensing
Operating temp range 18-24°C
Power 100-230 V AC, 50/60 Hz
Dimensions (H x W x D) 33 x 33 x 52 cm 84 x 94 x 61 cm 84 x 94 x 61 cm 84 x 94 x 61 cm
Part number 004-600 004-700 004-800 004-109

 

  Total Protein Immunoassay
Description Size Specification Size Specification Charge Specification
Sample required 0.3-1.2 µg lysate 0.6-1.2 µg 0.6-1.2 µg
Size or pI range 2-40, 12-230 and 66-440 kDa 2-40, 12-230 and 66-440 kDa Widest gradient ranges
from pI 3 to pI 10
Sizing CV <10% <10% <10%
Intra-assay CV <15% <15% <20%
Inter-assay CV <20% <20% <20%
Resolution
(± percent difference in MW)		 ± 15-20% for MW
< 20 kDa
± 10% for MW
> 20 kDa		 ± 15-20% for MW
< 20 kDa
± 10% for MW
> 20 kDa		 ± 0.1 pI units
Quantitation CV <20% <20% <20%
Dynamic range 2-3 logs up to 4 logs* 3 logs
Sensitivity ng Low pg Low pg
Capillary 5 cm, 100 µm, 400 nL

* Wes with HDR detection mode.