Simple Western systems

Gel-free, blot-free, hands-free

Simple Westerns™ let you separate and analyze proteins by size from 2-440 kDa either by immunoassay or total protein analysis. And there's even a charge-based assay if you're doing post-translational modification characterization. You'll get quantitative results, reproducibility that's spot on, and use less sample in the process. So what's the big difference between Simple Western and everything else? It's the only gel-free, blot-free, hands-free capillary-based immunoassay platform that integrates and automates the entire protein separation and detection process. So you'll have more time to get down to real science.

Wes

Wes

Size assays on up to 25 samples

Sally Sue

Sally Sue

Size assays on up to 96 samples

Peggy Sue

Peggy Sue

Size and charge assays on up to 96 samples

NanoPro

NanoPro 1000

Charge assays on up to 96 samples

Simple Western systems comparison chart

Which system is right for you?

Simple Western System Wes Sally Sue Peggy Sue NanoPro 1000
Simple Western size assays  
Simple Western charge assays    
Maximum samples per run 25 96 96 96
Run time for max samples (size) < 3 hours 14-19 hours 11-19 hours N/A
Run time for max samples (charge) N/A N/A 11 hours 11 hours
Sample cooling (size) N/A 10°C 10°C N/A
Sample cooling (charge) N/A N/A 3°C 3°C
Operating humidity range 20-60% relative, non-condensing
Operating temp range 18-24°C
Power 100-230 V AC, 50/60 Hz
Dimensions (H x W x D) 33 x 33 x 52 cm 84 x 94 x 61 cm 84 x 94 x 61 cm 84 x 94 x 61 cm
Part number 004-600 004-700 004-800 004-109

 

  Total Protein Immunoassay
Description Size Specification Size Specification Charge Specification
Sample required 0.3-1.2 µg lysate 0.6-1.2 µg 0.6-1.2 µg
Size or pI range 2-40, 12-230 and 66-440 kDa 2-40, 12-230 and 66-440 kDa Widest gradient ranges
from pI 3 to pI 10
Sizing CV <10% <10% <10%
Intra-assay CV <15% <15% <20%
Inter-assay CV <20% <20% <20%
Resolution
(± percent difference in MW)
± 15-20% for MW
< 20 kDa
± 10% for MW
> 20 kDa
± 15-20% for MW
< 20 kDa
± 10% for MW
> 20 kDa
± 0.1 pI units
Quantitation CV <20% <20% <20%
Dynamic range 2-3 logs up to 4 logs* 3 logs
Sensitivity ng Low pg Low pg
Capillary 5 cm, 100 µm, 400 nL

* Wes with HDR detection mode.