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Simple Plex assay for the detection of human Interferon gamma (IFN-γ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)
In this technical note, we demonstrate how diluting antibodies with Milk-Free Antibody Diluent when probing targets with a goat primary antibody delivers improved assay performance on the Milo Single-Cell Western platform.
This tech note describes how a minor modification in the antibody probing procedure used with a standard antibody probing chamber can improve probing uniformity and reduce technical noise in single-cell western assays.
Prior to initial use, and after running samples, ProteinSimple recommends cleaning the MFI flow cell using the specific guidelines contained herein.
SDS for Protein Normalization Assay Module for Jess
