For a list of peer-reviewed articles our products have been cited in, check out the publications page.
Learn how Simple Western can characterize full signaling pathways with little sample volumes, offering picogram-level sensitivity, and high-throughput analysis overnight.
ＨＤＲはHigh Dynamic Range(高いダイナミックレンジ)を表します。写真撮影においては最初に露出を変えながら複数の画像を撮影した後で、特殊なアルゴリズムを用いて、これらの画像のベスト部分を組み合わせて自動的に最良のイメージを作成します。 SimpleWestern®のＨＤＲも同様なアルゴリズムに基づいています。 ダイナミックレンジが広がると、貴重なサンプルを無駄にしたり、アッセイの最適化に多くの時間を取られることもなくなります。アッセイの最適化の時間を短縮するために、アッセイの検出ステップとデータ解析の両方を強化するＨＤＲ検出プロファイルを利用してWesのダイナミックレンジを拡大しました。
Block non-specific signal and prevent cross-reactivity when using anti-goat secondary antibodies with our Anti-Goat Detection Module for Wes, Peggy Sue and Sally Sue
If you’re experiencing primary antibody derived cross reactivity with the 230 kDa protein used as one of fluorescent standards, we’ve got a solution.
In this technical note we show you how this new assays works and give you a couple examples comparing Simple Western performance with traditional Western.
We know that your samples are usually the most precious part of an experiment. It can take a lot of time and resources that add to the expenses needed to grow and treat cells or months to raise and treat mice. But when researchers sit down to calculate the cost per data point they often forget to factor in the sample and focus more on things like buffers, gels, membranes, and antibodies. Plus, nobody wants to use up the limited amount of sample they have in one experiment just to have to take the time to make more samples when it's all about publishing or perishing.
Simple Western®, an immunoassay or total protein assay performed in a small capillary, has been the perfect solution when it comes to saving samples. And now we've just increased your sample savings by decreasing the amount of sample you need for each experiment.
Multiplexing two targets in one capillary with Simple Western doubles the number of data points per sample and increases the data quantitation accuracy when you normalize to biological loading control or system control.
When you're ready to start multiplexing, first optimize the primary antibodies separately to find the saturating dilution for each. Then combine them in Antibody Diluent 2 to detect the two targets in the same capillary. If the primary antibodies are raised in two different host species, you'll also need to combine two different anti-species secondary antibodies. But mixing Ready-To-Use (RTU) secondary antibodies will dilute them and cause the signal to decrease or become more variable because they're no longer saturating. We've got the situation under control with the 20X Anti-Rabbit HRP Conjugate.
HDR stands for high dynamic range and in photography it works by first taking a series of images with different exposures. Some cool math automatically combines the best parts of these images to create the best picture. HDR on Simple Western isn't all that different. And with a large dynamic range, you won't have to waste precious samples running assay optimization experiments. To reduce assay optimization time, we've expanded the dynamic range for Wes with a high dynamic range detection profile that enhances both the detection step in the assay and the data analysis.