Abby

The Next Generation Western Protein Analyzer

Abby is the next generation chemiluminescence western protein analyzer who moves your Westerns into the 21st century. She automates protein size separation and immunodetection while delivering analyzed results of 24 samples in 3 hours. Abby saves the time spent in the lab carrying out traditional Western blot steps such as antibody additions, incubations, and washes. She delivers picogram-level sensitivity through chemiluminescence detection and workflow automation. You'll never want to touch a traditional Western blot again!

Abby leapfrogs traditional western blot technology by allowing the flexibility to do more with your precious samples. Investigate total / phospho targets or detect multiple targets in consecutive immunoassays within the same capillary using RePlex. Enjoy the flexibility of probe choice, low volume sample requirement, more data points per sample, and Simple Western automation in the next generation of Western blot analysis. With Abby you can even normalize your data with total protein detection in the same capillary using RePlex. Abby, she’s the next generation Western super-hero.

Abby - Next Generation Western Protein Analyzer

 

ReThink, ReUse, RePlex!

RePlex™ with Abby enables you to run two immunoassays within the same capillary. You can also run an immunoassay and total protein analysis with the same sample! Get more protein characterization with less sample.

  • Quantify phosphorylated and non-phosphorylated protein expression levels
  • Normalize your immunoassay data using total protein detection within the same capillary
  • Significantly reduce time spent on your Western workflow
  • Lower your cost per data point

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RePlex™ with Abby instrument by running immunoassay and total protein analysis on the same sample

HOW CAN ABBY HELP YOUR WESTERN WORKFLOW?

I need faster time to data

Abby simplifies the steps involved in the Western workflow. Pipette your samples into her plate and let her do the rest! Abby automates protein separation, immunoprobing, detection and data analysis. In exchange for 30 minutes of easy experimental setup, you get the results of 24 fully-analyzed samples after 3 hours of hands-free run time. Speed up the time to data and your next publication.

Abby Automates Protein Separation, Immunoprobing, Detection and Data Analysis

I want better sensitivity from my Western

Working with low abundance targets or precious samples? Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample. Further conserve your precious sample by performing two chemiluminescence immunoassays in the same capillary using RePlex.

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Abby Chemiluminescence Delivers
Picogram Sensitivity
Abby Chemiluminescent Detection Provides Picogram Sensitivity

Reviewers are asking me to normalize to total protein

RePlex on Abby gives you an easy way to see if your samples contain a consistent total protein load by measuring all your proteins immobilized in the same capillary as your immunoassay. Increase confidence in your results through total protein normalization.

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Total Protein Normalization Using RePlex
Abby Allows You to Normalize Your Data with Total Protein Detection in the Same Capillary Using RePlex

I want to investigate total / phospho targets

Abby allows you to detect total / phospho targets within the same capillary using RePlex™.

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Total / Phospho Target Detection Using RePlex
Abby Demonstrates the Ability to Use Total and Phospho-specific Antibody Pairs in a Single RePlex Experiment

I need to conserve my precious sample

RePlex with Abby enables multiple targets to be probed within the same experiment, saving time and money while conserving precious sample. Multiplex multiple targets in consecutive immunoassays with chemiluminescent detection.

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Multiplex Multiple Targets in the Same Capillary

Abby Shows a Lane View of HSP60 and GAPDH Detected in the First Probe of a RePlex Immunoassay, and 14-3-3 and Park7 Detected in the Second Immunoassay

I need to spend less time in the lab

Are you behind in your research goals due to tedious Westerns protocols? Are you struggling to keep up with your sample workload because lab availability is limited? Let Abby move your Westerns into the 21st century! Enjoy automated Western steps and analyzed your data that can be accessed remotely three hours later.

Simple Western System
System Specification Abby Traditional Westerns
Maximum samples per run 25 25
Run time for mass samples (size assays) <3 hours
<5 hours using RePlex
2 days
Hands-on time 30 minutes 3.25 hours
Simple Western Applications

Cancer &
Immuno-Oncology

Simple Western Instrument Cancer Research Applications

 
 
SARS-CoV-2

Simple Western Instrument SARS-CoV-2 Research Applications

 
Cell & Gene Therapy

Simple Western Instrument Cell & Gene Therapy Research Applications

 
C&GT Western Blotting Solutions

Cell & Gene Therapy Western Blotting Solutions

 
Targeted Degradation

Targeted Degradation

 

SARS-CoV-2 Serology

SARS-CoV-2 Multi-Antigen Serology Module

 
Exosome

Exosome

Vaccine Development

Vaccines Development
HOW DO WESTERNS WORK ON ABBY?

Abby re-imagines traditional Westerns with automated western blot technology. Simple Western assays on Abby are automated, capillary-based immunoassays that overcome complexities inherent to traditional Western. Abby automates Western blotting protocol steps such as antibody additions, incubations, and washes. Having problems detecting your protein of interest? No problem! Her picogram sensitivity and total protein detection, combined with fully analyzed results in 3 – 5 hours, ensure that you'll never want to touch a traditional Western blot again!

Chemiluminescent detection: Are you working with low abundance proteins or have little sample to spare for analysis? Abby solves both problems with her chemiluminescent detection. Enjoy picogram-level sensitivity that enables you to get the most data out of your samples.

Total Protein normalization. Reviewers are moving away from accepting normalization by housekeeping proteins due to their expression variability across different sample types. RePlex with Abby provides a more reliable method of normalizing to total protein.

What does Simple Western data on Abby look like?

Abby’s fully analyzed results can be viewed in either lane or electropherogram views. You can use the lane view option to compare band intensity or quantitatively analyze protein size and concentration. Compare the expression of proteins of interest to total protein. Data analysis with Compass’s user-friendly software is quick and easy. Save graph and baseline options with run files and quickly annotate lane view results with Compass lane view features. Lane annotation with Compass 6.0 saves hours spent annotating data in other programs. Spend more time thinking about your next paper and less time interpreting the results.

Reimaging Western Data with Simple Western Data in Lane View with Abby

Reimaging Western Data with Simple Western Data in Electropherogram View with Abby

Specifications
Description Total Protein Specification Chemiluminescence Specification
Sample required 0.3-1.2 µg 0.6-1.2 µg
Volume required 3 µL/well
Size range Molecular weight (MW) ladder ranges from 2-440 kDa
Sizing CV <10%
Intra-assay CV <15%
Inter-assay CV <20% <20%*
Resolution (± percent difference in MW) ± 15-20% for MW <20 kDA
± 10% for MW >20 kDa
Quantitation CV <20% (total protein, chemiluminescence and fluorescence)
Dynamic range 2-3 logs 3-4 logs
Sensitivity ng Low pg
Capillary 5 cm, 100 µm, 400 nL
Runtime <3 hours
RePlex: 5 hours
Samples per run 13 or 25
Weight 21 kg
Dimensions (closed) 0.36 m H X 0.36 m W X 0.57 m D
Dimensions (open) 0.36 m H x 0.53 m W x 0.57 m D
Power US/CAN 120 V AC, 60 hz, 4.2 amps
Europe 240 AC, 50 Hz, 2.1 amps
Japan 100 AC, 50/60 Hz, 5.0 amps
Operating temperature 18-24 °C
Operating humidity 20-60% relative, non-condensing

* inter-assay CV is with system control
Percent peak area