Meet Abby, The Next Generation Western Protein Analyzer
Abby is the next generation chemiluminescence western protein analyzer who moves your Western workflow into the 21st century. She automates protein size separation and immunodetection while delivering analyzed results of 24 samples in 3 hours. Abby saves the time spent in the lab carrying out traditional Western blot steps such as antibody additions, incubations, and washes. She delivers picogram-level sensitivity using chemiluminescence detection and time through workflow automation. You'll never want to touch a traditional Western blot again!
Protein analysis comes with many challenges – labor-intensive protocols increase time to result and multiple hands on steps can increase user error and data variability.
Meet Abby, the next generation automated Western protein analyzer.
Abby automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps.
Just load your samples and reagents into the microplate, insert the plate and a capillary cartridge, and Abby does the rest.
She separates your protein by size, and precisely manages antibody additions, incubations, washes and even the detection steps.
Come back to fully analyzed, quantitative results in 3 hours.
With Abby you can even run two immunoassays in the same capillary using the RePlex module.
Abby’s chemiluminescent detection provides picogram level sensitivity, and with the new RePlex feature on Abby, you can run two immunoassays within the same capillary to get more protein characterization data from one sample.
RePlex also enables you to normalize your data with total protein detection in the same capillary.
RePlex with Abby delivers more data points per sample, saving time and money on consumables.
How Abby Performs Westerns
Simple Western immunoassays take place in a capillary.
Your sample, separation matrix, stacking matrix, antibodies and reagents are loaded automatically from a specially designed plate.
Abby begins by aspirating the separation matrix and then the stacking matrix into each capillary.
Next your protein sample is loaded, and capillaries are lowered to make contact with running buffer.
Voltage is applied to separate the proteins by molecular weight.
Once the separation is complete, UV light immobilizes the proteins covalently to the capillary wall and the matrix is cleared from the capillary.
With proteins now ready for immunoanalysis, Abby starts the immunoprobing process.
During immunoprobing, the samples are incubated with a primary antibody, followed by an HRP conjugated secondary antibody, and finally, a chemiluminescent substrate.
The chemiluminescent reaction is recorded by a CCD camera in a series of images over time.
With RePlex, Abby will then remove all antibodies from the first immunoprobe with a proprietary reagent leaving the immobilized samples ready for a second round of immunoprobing.
With the RePlex feature on Abby, you can run two immunoassays within the same capillary to get more protein characterization data from one sample, and even normalize your data with total protein expression data in the same capillary.
To learn more about the RePlex assay, visit the RePlex product page.
Analyzing Results with Abby
After your run, analysis is a breeze.
Want to identify whether a protein is present or absent?
Abby gives you the qualitative western data you are used to seeing.
Even better, she’ll quantitate the data for you too.
With a few clicks you’ll be analyzing immunoassay-like standard curves and precisely quantifying your protein.
Abby also includes protein normalization: she will compare total protein loads across your samples, and normalize the signals, increasing your confidence in your data interpretation.
Abby: she’s the next generation automated Western super-hero.
Learn more about Abby by ProteinSimple, a Bio-Techne brand, by visiting the Abby instrument page .