Advanced Western Blotting Solutions for Cancer Research

Simple Western requires only a few microliters of LCM sample to generate multiple data points. Simple Western also has the sensitivity to analyze low abundance proteins that are difficult to detect by traditional Western blot. And it’s all wrapped up in a simple workflow that minimizes your hands-on time. In this application note, we show two examples of how Simple Western was ideally suited for the analysis of LCM samples. Request pricing or more information on Simple Westerns.

Milo can analyze samples which contain as low as 10,000 single-cells so you no longer have to collect millions of cells to analyze protein expression. The number of cells captured scales with the number of cells loaded so even lower abundance samples are possible. Milo can also characterize highly enriched FACS-sorted cell populations which don't contain enough cells to analyze with other techniques. Request pricing or more information on Single-Cell Westerns.

Tumor heterogeneity poses a significant challenge to the development of effective cancer therapies. Further, the tumor microenvironment creates a complex ecosystem of diverse tumor and immune cell types. Methodologies like flow cytometry have limitations such as off-target binding of variants/ isoforms and difficulties in finding validated antibodies. In this application note, Simple Western and Single-Cell Western systems partner to get you critical answers on the immune cell populations found in the tumor microenvironment offering both population level views and single-cell level perspectives on cell subtypes. Request pricing or more information on Simple Westerns and Single-Cell Westerns.

Quantitative measurements of protein expression are extremely difficult with traditional western blotting due to variability during membrane transfer. Further, simultaneous phospho-protein and total protein measurements on the same sample are challenging on conventional western blots due to loss of protein and non-uniformity during stripping & reprobing. With Simple Westerns, proteins are immobilized after separation, reducing variability and enabling quantitative measurements of protein abundance. Further, multiplexing is easy using multiple detection channels or RePlexing cycles to enable phospho/total measurements in the same capillary. Total protein measurements can also be made on the same sample for more accurate and robust normalization. Request pricing or more information on Simple Westerns.

The Milo™ Single-Cell Western system helps detect discrete cell signaling states by measuring multiple phospho-proteins in individual cells within a population.  Because cells are lysed in Single-Cell Westerns, membrane permeabilization is not required and both intracellular and intranuclear proteins are accessible for antibody probing, making it possible to use phospho- antibodies validated for western blotting and simplifying some challenging intracellular flow assays. This application note demonstrates how Milo adds single cell resolution and heterogeneity information to cell signaling studies traditionally done with conventional western blotting and simplifies challenging phospho-flow assays done with flow cytometry. Request pricing or more information on Single-Cell Westerns.