Your Complete Protein Analysis Solution
Jess automates traditional Western blotting while maximizing multiplexing with multiple detection channels. Automation of protein separation and immunodetection eliminates many of the tedious, error-prone steps of traditional Western blotting which limit data quality. Just load your samples and reagents into the microplate and Jess separates your proteins by size, and precisely manages antibody additions, incubations, washes and even the detection steps. Come back to fully analyzed western blotting results on 24 samples in 3 hours. Go further with multiplexing—her fluorescent detection channels and RePlexing capabilities get you all the information you need on your samples in one run. Jess automated western blot system—she's like multiplexed Western blot meets ELISA in one.
Simple Western assays on Jess are automated, capillary-based immunoassays that solve many of the challenges that come with traditional Western blots. Jess automates Western blotting workflows, integrating all assay steps from protein separation, immunoprobing, detection and analysis of data.
Jess gives you four different ways to analyze proteins, giving you flexibility in your western blotting workflows.
- Fluorescent detection: Why bother stripping and reprobing? Maximize your time and sample, and get the information you need in one shot, with multiplexing.
- Chemiluminescent detection: Working with low abundance targets or precious samples? Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample.
- Protein normalization. Jess gives you several easy and robust ways to see if your samples contain a consistent protein load and normalize your immunoassay data to total protein content rather than a loading control. Don't strip & reprobe, RePlex your Jess assay by removing your first set of antibodies and performing a sequential total protein assay in the same capillary. Alternatively, use Jess's fluorescent Protein Normalization assay. Just load her in-capillary protein normalization reagent into her assay plate and she'll take care of the rest. Her proprietary fluorescent reagent measures proteins immobilized in the same capillary as your immunoassay. The result of either approach? You can quickly see if your samples contain a consistent protein load, identify experimental setup and user errors, and effectively normalize expression of your target protein to get accurate and consistent data, giving you the confidence you need in your results. Best of all, Jess's fluorescent detection capabilities enable two-color protein detection for multiplexing on top of protein normalization.
- Blot imaging: Still doing traditional western blots? Snap! Get the picture with Jess's in-built blot imaging system.
Simple Western immunoassays take place in a capillary. Your sample, separation matrix, stacking matrix, antibodies and reagents are loaded automatically from a specially designed plate. Jess begins by aspirating the separation matrix and then the stacking matrix into each capillary. Next your sample is loaded, and capillaries are lowered to make contact with running buffer. Voltage is applied to enable separation by molecular weight. Once the separation is complete, UV light immobilizes the proteins to the capillary wall. With proteins now immobilized and the matrix cleared of the capillary, Jess starts the immunoprobing process, first with incubation with the primary antibody, followed by a secondary HRP conjugate, and finally chemiluminescent substrate. The chemiluminescent reaction is recorded by a CCD camera in a series of images over time. In just 3 hours you'll have quantitative, size-based data ready for analysis.
Her fluorescent detection capabilities enable two-color protein detection for multiplexing. During the immunoprobing process samples are incubated with the primary antibody, followed by infrared or near-infrared fluorescent secondary-tagged antibodies. Excitation of the fluorphores releases photons and the emission spectra is detected by wavelength sensors and recorded by a CCD camera in a series of images over time. In just 3 hours you'll have multiplexed, quantitative, size-based data ready for analysis.
Protein normalization has never been easier with Jess. Jess gives you several easy and robust ways to normalize your immunoassay data to total protein content rather than a loading control. Choose between RePlexing with an immunoassay followed by a sequential total protein assay in the same capillary or Jess's fluorescent Protein Normalization assay. To run her Protein Normalization assay, just load the Protein Normalization Regent into a row of wells on the plate and Jess will take care of the rest. The fluorescent-labeled reagent reacts with amines on proteins, enabling a between capillary comparison of protein load. Best of all, Jess's fluorescent detection capabilities enable two-color protein detection for multiplexing on top of protein normalization. With either approach, now you can effectively normalize target protein expression data, produce accurate and consistent Western blotting data, and mitigate experimental setup and user error.
What does Simple Western data on Jess look like? At the end of your run, use the lane view option to compare band intensity or dive deep for fully quantitative analysis of protein size and concentration. Dive deeper into quantitative analysis to compare protein expression changes and analyze protein isoforms or size changes. Want to analyze expression changes between samples or compare runs? Our protein normalization will give you the confidence you need in your analysis.
|Sample required||0.3-1.2 µg||0.6-1.2 µg||2-4 µg||0.6-4 µg|
|Volume required||3 µL/well|
|Size range||Molecular weight (MW) ladder ranges from 2-440 kDa|
|Resolution (± percent difference in MW)||± 15-20% for MW <20 kDA
± 10% for MW >20 kDa
|Quantitation CV||<20% (total protein, chemiluminescence and fluorescence)||N/A|
|Dynamic range||2-3 logs||3-4 logs||3-4 logs||1 log|
|Sensitivity||ng||Low pg||High pg||ng|
|Capillary||5 cm, 100 µm, 400 nL|
|Runtime||<3 hours||<4 hours with immunoassay|
|Samples per run||13 or 25|
|Dimensions (closed)||0.36 m H X 0.3 m W X 0.57 m D|
|Dimensions (open)||0.36 m H x 0.53 m W x 0.57 m D|
|Power||US/CAN 120 V AC, 60 hz, 4.2 amps
Europe 240 AC, 50 Hz, 2.1 amps
Japan 100 AC, 50/60 Hz, 5.0 amps
|Operating temperature||18-24 °C|
|Operating humidity||20-60% relative, non-condensing|
*inter-assay CV is with system control
**percent peak area