Why do Protein Normalization?

Planning on publishing your research findings? Then you know scientific journals and peer reviewers need to see some type of total protein characterization that validates your data. They're also asking scientists to move away from housekeeping proteins (HKP) because their expressions are inconsistent and unreliable. That's where protein normalization (PN) assays on Jess come in. They give you a more consistent, robust way to determine protein content.

Protein Normalization vs Housekeeping Proteins

Graph - protein normalization vs β-Actin

Comparative total protein data and the expression of the housekeeping protein β-actin in six human whole tissue lysates loaded on an equal protein basis (0.3 mg/mL).