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Sally Sue

So frugal, so accurate, so easy

Sally Sue™ lets you separate and analyze proteins by size from 2-440 kDa either by immunoassay or total protein through automated western analysis. Got small sample volumes or starting materials? No problem. She uses as little as 0.2 µg/µL of protein in just 5 µL of sample, and runs up to 96 samples in one experiment. If you wish you could do more, Sally Sue gets you there!

Automated Western with Sally Sue, a Simple Western Instrument

How Can Sally Sue Help You?

My sample is precious and I don't have enough for other methods like western blot

Sometimes you wish you could do more with your small and precious sample. Sally Sue makes the most of your limited sample and is our most frugal automated Simple Western systems. She can run up to 96 data points in one overnight run with as little as 5 µL of sample. Sally Sue can also utilize that sample to its maximum potential by allowing you to interrogate those few microliters eight times, such as when analyzing an entire cell signaling pathway in a single run. Imagine all the data you can get from your sample using a single Simple Western run on Sally Sue!

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Automated Western Results using Small Sample Sizes with Peggy Sue, a Simple Western Instrument

I have too many samples and need higher throughput protein characterization

Simple Western assays on systems like Sally Sue offer the right throughput for your needs. Sally Sue will process up to 96 data points per run in eight cycles of 12 data points each, ideal for getting a lot of data in a single run. Automated western analysis on Sally Sue provides all the throughput you need with minimum hands-on time.

High-Throughput Simple Western Analysis on Many Samples with Sally Sue

Sally Sue can analyze an entire cell signaling pathway in a single run. HeLa cells were left untreated or treated with TNFa to stimulate the NF?B signaling pathway. Using only 5 µL/well (1 µg/µL) of either a whole cell lysate (WC) or nuclear extract (NE) from those cells, Sally Sue could quantitate seven different proteins from the pathway plus a loading control (αTubulin).

I am in a race to discover and I need my western data faster

If analyzing 96 data points overnight is not impressive enough with Sally Sue's automated western analysis, maybe we should add that also includes data analysis. Set up your automated western protocol, start your run in the afternoon, and come back the next morning to find your test results with molecular weight and signal quantification automatically calculated for each sample. If your time is as precious as your samples and you need results faster, we will help you win the race to discovery with Sally Sue.

Click on the clock to the right to see how Simple Western expedites time to data over traditional western blotting workflows.

My current western blot method is not reproducible

How reproducible will your current immunoassay be in analyzing 96 data points? With Sally Sue's automated western capabilities you get intra-assay CV<15% and inter-assay CV<20%. How's that for reproducibility?

Reproducible Results with Automated Westerns using Sally Sue

Detection of PI3 Kinase in a serial dilution of HeLa lysate (1000 ng/µL — 37 ng/µL). All eight cycles showed similar results (CV <15%). Linearity of the dilution series (R2=0.989) is maintained even at very low protein concentrations.

Simple Western Applications

Cancer &

Simple Western Instrument Cancer Research Applications


Simple Western Instrument SARS-CoV-2 Research Applications

Cell & Gene Therapy

Simple Western Instrument Cell & Gene Therapy Research Applications

C&GT Western Blotting Solutions

Cell & Gene Therapy Western Blotting Solutions


Targeted Protein Degradation

Targeted Degradation

SARS-CoV-2 Serology

SARS-CoV-2 Multi-Antigen Serology Module



Vaccine Development

Vaccines Development




Sample Required 0.3-1.2 µg 0.6-1.2 µg
Volume Required 3-5 µL/well 3-5 µL/well
Size Range Molecular weight (MW) ladders range from 2-440 kDa Molecular weight (MW) ladders range from 2-440 kDa
Sizing CV <10% <10%
Intra-assay CV <15% <15%
Inter-assay CV <20% <20%
(± percent difference in MW)
± 15-20% for MW <20 kDa
± 10% for MW >20 kDa
± 15-20% for MW <20 kDa
± 10% for MW >20 kDa
Quantitation CV <20% <20%
Dynamic Range 3 logs 3 logs
Sensitivity ng Low pg
Capillary 5 cm, 100 µm, 400 nL