Sally Sue video
Sally Sue Simple Western
The gel-free, blot-free, hands-free Simple Western is here! Sally Sue is an automated immunoassay using capillary electrophoresis. Sample loading, size-based separation, immunoprobing, washing, detection and quantitative data analysis are all done automatically, giving you a complete, walk-away solution. There are no messy gels, no transfer tanks or blots, and no film or imaging required. It’s just simple!
About Sally Sue Automated Immunoassays
The gel free, blot free, hands-free, Simple Western is here.
Brought to you by ProteinSimple a Bio-Techne brand.
The Simple Western is an automated immunoassay using capillary electrophoresis.
There are no messy gels, no transfer tanks or blots, and no film or imaging required.
It's really just simple.
Meet Sally Sue. She can do up to 96 Simple Westerns in one experiment for you.
All you do is prepare your samples, push start on the software, and let Sally Sue do the rest for you.
When the experiment is done, you'll come back to fully analyze data that is more quantitative and more reproducible.
Sally Sue and Western Blots
You're probably wondering exactly how Sally Sue does Simple Westerns.
Well, everything happens inside of a capillary.
Sally Sue picks up 12 capillaries at a time for each cycle.
She does up to 8 cycles for a total of 96 Simple Westerns.
Sally Sue will load samples, antibodies, and reagents into each capillary from a 384-well plate.
She begins by first loading the separation matrix and then a stocking matrix into each capillary.
Sally Sue then loads your protein lysate.
The capillaries are placed into the separation tray where a voltage is applied across each capillary to enable separation by molecular weight.
Once the separation is complete, the capillaries are exposed to ultraviolet (UV) light which immobilizes your proteins to the capillary walls.
This allows Sally Sue to perform an immunoassay within the capillary by first incubating with primary antibody and then a Horseradish Peroxidase (HRP) conjugated secondary antibody followed by a chemiluminescence substrate.
This chemiluminescence reaction gives off light that is read by a CCD camera.
Your quantitative results are automatically generated for you and reported by electropherogram or lane view.
Simple Westerns simply offer you more.
Get separation by size, target identification, quantitation, and high throughput all from Sally Sue.
Learn more about the gel free, blot free, hands-free, Simple Western by ProteinSimple, a Bio-Techne brand, by visiting Sally Sue instrument page.