Rapid Characterization of Human Immune Response to SARS-CoV-2 Antigens Using Simple Western

SARS-CoV-2 Multi-Antigen Serology Module for Jess/Wes

The SARS-CoV-2 Multi-Antigen Serology Module (SA-001) for use with Jess and Wes automated western blotting systems enables the detection of human IgG antibodies in human serum or plasma reactive against multiple SARS-CoV-2 antigens. With this antigen down serology assay, you can quantitatively characterize serum or plasma human IgG antibodies reactive against recombinant Nucleocapsid Protein (N), S1 receptor binding domain protein (RBD), S1 subunit full length, S2 subunit full length, and Spike (S1+S2) viral antigens within one 3-hour run! Learn more about the benefits of the SARS-CoV-2 serology assay that include the ability to:

  • Quantify differences in patient serum antibody binding patterns using 5 different SARS-CoV-2 antigens in each sample
  • Speed up your COVID-19 studies with minimal set-up time, hands-free operation and fully analyzed results on 24 samples in 3 hours.
  • Access your results remotely to minimize time in the lab

Simple Western SARS-CoV-2 serology assay demonstrates differential patient reactivity to N, S1 RBD, S1 full length, S2 full length, and Spike (S1+S2) COVID-19 viral antigens

Quantification of percent peak area for SARS-CoV-2 antigens detected by PCR positive human serum

Figure 1: Simple Western SARS-CoV-2 serology assay demonstrates differential patient serum reactivity to N, S1 RBD, S1 full length, S2 full length, and Spike (S1+S2) COVID-19 viral antigens. Quantification of percent peak area for SARS-CoV-2 antigens detected by PCR positive human serum.

Simple Western SARS-CoV-2 serology assay demonstrates differential patient reactivity of PCR positive human plasma samples to N, S1 RBD, S1 full length, S2 full length, and Spike (S1+S2) COVID-19 viral antigens

Figure 2: Simple Western SARS-CoV-2 serology assay is validated for human serum and plasma. Differential patient reactivity of PCR positive human plasma samples to N, S1 RBD, S1 full length, S2 full length, and Spike (S1+S2) COVID-19 viral antigens.

Workflow schematic of the Simple Western SARS-CoV-2 serology assay

Figure 3: Workflow schematic of the Simple Western SARS-CoV-2 serology assay.  Learn more about how SARS-CoV-2 Serological Assays on Simple Western work.

I Need a Fast, Confirmatory Assay for My SARS-CoV-2 ELISA

Western blot-based serology assays are commonly used to confirm ELISA-based serology tests. The SARS-CoV-2 Multi-Antigen Serology Module for Simple Western demonstrates excellent agreement with the recently released Quantitative SARS-CoV-2 IgG Antibody RUO ELISA Kit from Kantaro Biosciences, Powered by R&D Systems (96% positive percent agreement and 99% negative percent agreement). The fully automated Simple Western serology assay is a powerful orthogonal assay that provides characterization of patient immune responses to all five SARS-CoV-2 antigens in only three hours. Read the app note to learn more.

Benchmarking Against Kantaro RBD ELISA
    Kantaro ELISA RBD
Positive Negative
SARS-CoV-2 Multi-Antigen Serology Module for Jess/Wes Positive 72 1
Negative 3 99
  Total 75 100
  Positive Percent Agreement (PPA) 96%  
  Negative Percent Agreement (NPA) 99%  

Figure 4: Positive Percent Agreement (PPA) and Negative Percent Agreement (NPA) study benchmarking the SARS-CoV-2 Multi-Antigen Serology Module against the Kantaro RBD ELISA.

Faster time to data with exceptional sensitivity!

The SARS-CoV-2 Multi-Antigen Serology Module for Simple Western™ provides an immune fingerprint of serum antibodies against five antigens commonly associated with SARS-CoV-2 infection in only three hours.  As little as 1 µL of serum can be utilized for each patient sample. Titer studies have shown sensitivity down to 1:138,240 with R2 linearity >0.99 across the dilution series. Download the app note to learn more about a serial dilution study that demonstrates both assay linearity and unparalleled sensitivity to SARS-CoV-2 antigens.

SARS-CoV-2 serial dilution study demonstrating assay sensitivity and linearity

Linearity of SARS-CoV-2 Antibodies in Human Serum with dilution series run from 1:40 to 1:138240

Figure 5: Serial dilution study demonstrating assay sensitivity and linearity. Dilution series run from 1:40 to 1:138240. Not visible in the electropherogram are 1:17280, 1:34560, 1:69120, and 1:138240

 

 

Applications

 
Cancer

Simple Western Instrument Cancer Research Applications

 
SARS-CoV-2

Simple Western Instrument SARS-CoV-2 Research Applications

Cell & Gene Therapy

Simple Western Instrument Cell & Gene Therapy Research Applications

Targeted Degradation

Targeted Degradation

 

SARS-CoV-2 Serology

SARS-CoV-2 Multi-Antigen Serology Module