Simple Plex for Cell Therapy
Automated Immunoassays for Cell Therapy Process Development
Automated Simple Plex assays on the Ella platform offer high quality immunoassay data and unparalleled efficiency for your cell therapy workflow.
- Ella reduces the manual operation of an immunoassay by 80 percent. Simply dilute samples and add them to the cartridge.
- With a total time to result of just 90 minutes, Ella gives you results in a fraction of the time of a manual assay.
- Sample volume requirements of 25 µL or less and triplicate measurements from every sample well reduce consumption of your precious samples.
- Measure viral titers and characterize transduced cells with Simple Plex assays designed to meet your needs.
Consistent and Reliable
Simple Plex assays on Ella deliver the same data regardless of instrument, operator or location.
- Volume independent assays that are robust to how much sample is added
- Factory calibrated cartridges minimize inter-lot variability allowing you to get the same measurement anywhere
- Reduction in manual steps eliminates operator error
Testing across 3 sites, 11 users and 9 Ella instruments. Four different assays were processed for CCL2, IL-6, TNF α and VEGF-A. Eight unique serum samples with 2 controls for a total of 704 answers.
Solutions for CAR-T Manufacturing
The manufacturing of CAR expressing T-cells is a multistep process that requires rigorous product characterization at various points throughout the workflow. Ella gives you the critical information you need faster, with superior sensitivity and reproducibility that supports regulatory compliance. Accelerate your process development with automated Simple Plex assays for viral titer and T-cell characterization.
Viral Titer Assays
Accurate detection and quantitative measurement of viral titer in cell cultures is critical for implementation of effective gene transfer treatment strategies. Simple Plex assays are available to quantify viral titers of both lentivirus and adeno associated virus capsids.
Simple Plex HIV p24 Lentiviral Titer Assay
The Simple Plex assay for the detection of human HIV-1 Gag p24 combines high throughput with superior accuracy and precision to enable rapid quantification of lentiviral capsid proteins.
Partners in AAV Titration Assays
To bring you the best quality immunoassays, we’ve partnered with the industry leader in Adeno-associated virus quantitation, PROGEN.
The Simple Plex AAV2 Assay
The Simple Plex AAV2 assay gives you a fast, sensitive and reproducible method for the reliable titration of intact AAV2 WT virions, AAV2 recombinant virions, or assembled and intact empty AAV2 capsids—all critical viral quantifications to help a safe and reliable gene transfer. The AAV2 Titration assay uses the A20R antibody from industry partner PROGEN to detect a conformational epitope not present on unassembled capsid proteins. PROGEN antibodies are among the most published antibodies in biomedical and cell biology literature.
| Simple Plex AAV2 Assay Specs | |
|---|---|
| LLOQ | 3.41 x 106 capsids/mL |
| ULOQ | 1.3 x 1010 capsids/mL |
How Does the Simple Plex AAV2 Assay Compare with the PROGEN AAV2 ELISA?
In this application note, we demonstrate assay comparability between the Simple Plex AAV2 assay and the PROGEN AAV2 Titration ELISA (PRATV) to show that you get the same high-quality data you would expect from the PROGEN ELISA with an automated, streamlined workflow.
Multianalyte Measurements for T Cell Characterization
Multianalyte cartridges on Ella allow you to take multiple biomarker measurements in parallel rather than in multiplex. This ensures the highest quality data since all assays are run in a discrete environment. Combine any four analytes from our menu into custom panel for your T cells.
| Simple Plex Assays for T Cell Characterization | ||
|---|---|---|
| Granzyme B | IL-4 | IL-17A |
| IFN-γ 3rd Gen | IL-5 | TGF-β1 |
| IL-2 | IL-10 | TNF-α 2nd Gen |
See the full Simple Plex assay menu, learn more about how Ella works or talk to one of our Immunoassay specialists about Cell and Gene Therapy solutions for your lab.
Bio-Techne's Tools for Cell and Gene Therapy Development
Bio-Techne offers a diverse portfolio of products and services designed to accelerate and simplify your cell and gene therapy workflow. From GMP proteins and small molecules to state-of-the-art analytical instrumentation, Bio-Techne is committed to providing high quality innovative solutions. Learn more about Bio-Techne’s cell and gene therapy offerings.
COVID-19
Inflammation
Cancer
Neuroscience
Cell Therapy
Bioprocess Dev.
Preclinical Res.
Viral Titer Assay
| 16x1 | 32x1 | 16x2 | 16x3 | 16x4 | 32x2 | 72x1 | 32x3 | 32x4 | 32x5 | 32x6 | 32x7 | 32x8 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Samples | 16 | 32 | 16 | 16 | 16 | 32 | 72 | 32 | 32 | 32 | 32 | 32 | 32 |
| Plex | 1 | 1 | 2 | 3 | 4 | 2 | 1 | 3 | 4 | 5 | 6 | 7 | 8 |
| Replicates | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 2 | 2 | 2 |
| Reportable Results | 16 | 32 | 32 | 48 | 64 | 64 | 72 | 96 | 128 | 160 | 192 | 224 | 256 |
Single Plex
Multianalyte
Multiplex
Customizable
Stocked
