Superplex Your Western Blots
Assay in chemiluminescence, 2-color fluorescence, total protein, and protein normalization at the same time.
What is Superplexing?
Jess' "Superplexing" goes so far beyond what's ever been done before with multiplexing on Western blot and other protein analysis systems, we just had to call it something else.
Western blots are the standard laboratory method for detecting and analyzing total proteins and protein normalization. They are based on a proven methodology with extremely high accuracy and are a fundamental technique used in labs and research studies globally. Here at ProteinSimple, we are taking Western blots to the next level with instruments which conduct multiple tests for targets and parameters simultaneously.
Only on Jess.
Superplex Western Assay Capabilities
Multiplex up to three channels to detect protein targets
When you Superplex with Jess, you've got one chemiluminescence and two-color fluorescence (NIR and IR) channels to detect protein targets in your Western blot tests. There's no one way to go—just mix and match to fit your needs for a specific sample or project. And you'll never have to give one up to run total protein because that happens on a totally different channel.
Simple Western size assays give you all the flexibility you need to detect multiple protein targets using Western blot testing. Use ultra-sensitive chemi assays for targets like phosphorylated proteins. When you want to do traditional multiplexing to look at two proteins with the same molecular weight (MW), just use NIR and IR fluorescence to see both. Got a third target at the same MW? Do a 3-plex and spot that one with chemi while you're looking at the other two with fluorescence. You can also look at multiple targets with different molecular weights anytime you need to. Plan on publishing your data or just want a little extra confidence in your results? Just have Jess run a protein normalization assay for you at the same time, don't even bother with HKPs. And when you need to kick that article out fast, Superplexing on Jess clocks in with at least 27 data points/hr with protein normalization on 25 samples within 3.5 hours.
Multiplex Western Blotting Pros and Cons
Use Superplexing for better results and fewer drawbacks
Until now, multiplexing Western blots was the only way to go—but we all know the roadblocks that come with it. Superplexing lets you keep everything you like about multiplexing now and eliminate what you don't.
The Superplex Workflow and Data on Jess
Getting more Western blot data points in less time
When you Superplex with Jess, one tiny 3 µL sample gives you at least 4 data points. Not to mention the time you save with your Western blots by doing four things at once, and that it only takes 3.5 hours to do them. But those aren't the only pros of Superplexing. Because each of those data points gets captured in the same capillary, you're not spending time comparing, infering and interpreting data across runs, samples, or lanes either. That means you get to make more informed, confident conclusions about your Western blot test results.
Setting up the Superplex test on Jess is simple. Load samples, antibodies and reagents into a plate. Then load the plate a capillary cartridge into the Jess instrument. She takes care of everything from there. Compass for Simple Western software automatically analyzes the data when she's done. That includes normalizing target data, so no need to bust out the pencil and TI-86.
Need to crunch the numbers for external normalization or to plot target protein expression against the total amount of protein in each capillary? No problem. Compass lets you export your data in a format that’ll let you do that.