For a list of peer-reviewed articles our products have been cited in, check out the publications page.
To characterize the efficacy of Degrader molecules, researchers generally run dose response curves by way of traditional SDS-PAGE Western blotting methods. Simple Westerns let you separate and analyze proteins by size (or charge) from 2 kDa to 440 kDa in just 3 hours.
CRISPR/Cas9 has become a revolutionary tool for precise genome editing in a wide variety of prokaryotes and eukaryotes, and multiple Cas9 variants have been engineered to further broaden its functionality. Purity, heterogeneity and stability are critical properties in the biophysical characterization, chemical modification and structural investigation of Cas9 and its variants. At the forefront of techniques to monitor these properties are capillary electrophoresis sodium dodecyl sulfate (CE-SDS) and capillary isoelectric focusing (cIEF). Maurice™ from ProteinSimple enables both CESDS and imaged cIEF (icIEF) in a single unit, with several key advantages over other CE-SDS and cIEF systems, such as ease-of-use, high data quality, and speed.
The power of Simple Western platforms, like Peggy Sue™, Sally Sue™, and NanoPro 1000™, has been harnessed in many fields of research including cancer, drug discovery, stem cells and regeneration, and cell and gene therapy. Below are just a few examples of how researchers are using Simple Western assays to make important scientific breakthroughs.
Simple Western Charge assays on Peggy Sue™ or NanoPro 1000™ analyze up to 96 native, non-denatured samples in a fully automated fashion, and the data generated are reproducible and quantitative. Simple Western Charge assays use as little as 0.2 μg/μL of protein in just 5 μL of sample, and those few microliters can be interrogated up to eight times! This means you’ll get a large amount of data from a very small sample size.
ProteinSimple offers a range of research tools to study the biological processes that accompany neurological/neurodegenerative disease.
In this application note, we’ll show you how Wes™ and Milo™ partner to get you critical
answers to 1) what type of immune cell populations are present in a sample and then 2) what percentage of cells in that sample
make up a specific immune cell subtype.
Charge heterogeneity is a critical quality attribute in the development of ADCs and must be carefully monitored throughout the development pipeline. For this purpose, ProteinSimple offers iCE3™ and Maurice™ systems, which are powerful tools to analyze the charge heterogeneity of your ADCs.
Protein aggregation and the formation of subvisible particles can significantly impact the safety and efficacy of biopharmaceuticals. Therefore, protein aggregation and subvisible particle content must be carefully monitored during in-process and final product development. ProteinSimple offers Micro-Flow Imaging™ (MFI), a powerful system to analyze protein aggregates and other subvisible particles in your samples.
We enable cutting-edge research to uncover the role of proteins in cancer and provide novel approaches to develop and
analyze protein-based therapeutics. We empower you to make your next discovery by eliminating common protein analysis
Identification and monitoring of biomarkers related to T cell activation and associated cytokine release syndrome (CRS) will be necessary to fully realize the immense potential of chimeric antigen receptor (CAR) T cell therapy. Such biomarkers could be used to guide clinical development of candidate therapies1, provide mechanistic insight into patterns of resistance2, and evaluate strategies to mitigate toxicity3. Establishment of predictive biomarkers is critical to maximizing therapeutic benefits of immunotherapy4. Correlating biomarkers with clinical evidence will facilitate early identification of patients at risk of developing CRS and enhance efforts to safely deliver CAR T therapy5.