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WES HAS BEEN DISCONTINUED AS OF JULY 30TH, 2021. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026.
If you are interested in Simple Western’s Wes Instrument, please view the comparison of our Jess and Abby instruments below. These options feature all the same benefits as Wes with the addition of multiplexing.

 Simple Western Jess Instrument: Automated Western Blotting System with Chemiluminescence, Fluorescence and RePlex


Size assays on up to 25 samples
Chemiluminescence and Fluorescence
Includes RePlex
Includes Stellar High Sensitivity NIR / IR

Simple Western Abby Instrument: Automated Western Blotting System with Chemiluminescence and RePlex Features.


Size assays on up to 25 samples
Includes RePlex

Which system is right for you?

Simple Western System Jess Abby Wes
Simple Western size assays
Simple Western charge assays      
Maximum samples per run 25 25 25
Run time for max samples (size) < 3 hours < 3 hours < 3 hours
Run time for max samples (charge) N/A N/A N/A
Sample cooling (size) N/A N/A N/A
Sample cooling (charge) N/A N/A N/A
Operating humidity range 20-60% relative, non-condensing
Operating temp range 18-24°C
Power 100-230 V AC, 50/60 Hz
Dimensions (H x W x D) 33 x 33 x 52 cm 33 x 33 x 52 cm 33 x 33 x 52 cm
Part number 004-650 004-680 004-600

Simple Westerns just got even simpler

Wes™ lets you separate and analyze proteins by size from 2-440 kDa. He also gets you down to pg-level sensitivity with just 3 µL of starting material. Got a lot of samples and no time? No problem. Unlike traditional western blots, Wes runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. Don't wait to discover, get going today with Wes!

Automated Western Blotting with Wes

How Can Wes Help You Automate Your Western Blot Technique?

I have too many samples and need higher throughput protein characterization

Our Simple Western systems offer the right throughput for your needs. Whether you are looking to process up to 24 samples per run or just 13, we have an instrument for you. Screen and optimize assays in less time than traditional western blotting. All the throughput you need with minimum hands-on time.

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  Time to Result Throughput Your Freedom
Traditional Western Blotting 24 HOURS 20 CONFINED

I need a method that is quantitative

Whether you want to do absolute or relative quantitation of your target, you need to reliably be able to detect signal saturation and work within the linear dynamic range of your assay. With HDR detection profile and analysis Wes gets you up to 6 logs of dynamic range. This means less time optimizing to get within the linear detection range of your assay.

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Automated Western Blotting and Relative Chemiluminescence Measurements

My sample is precious and I don't have enough for other methods

Traditional immunoassays, like western blot, often require too much of your precious sample. You end up having to pool samples together or be content with getting just a few data points per experiment. With Wes you only need 3 µL of sample per capillary, so you can maximize the data you get per experiment. Imagine all the data you can get from your sample!

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"With Wes, we can do multiple assays with one sample collection."

— Mary Howell, Laboratory Coordinator, Department of Internal Medicine, East Tennessee State University

Multiple assay with one sample with automated western blotting

I am in a race to discover and I need my data faster

Tired of being handcuffed to your bench for hours tending to your gels and blots? Or maybe you are waiting in line for the dark room to free up? If your time is as precious as your samples and you need results faster, we will help you win the race to discovery with Wes—our gel-free blot-free, hands-free Simple Western instrument.

Click on the clock to see your time savings.

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My current method is not reproducible

Are your assays lacking the reproducibility and reliability that you need to be confident in your research? With Wes, you can achieve intra-assay CV <15%. How's that for reproducibility?

Reproducible Results with Automated Western Blotting

%CV for ERK1 = 9.4% (n=24)

The environment matters to me

Meet Jamie Boisvenue from the Department of Pediatrics, University of Alberta Faculty of Medicine & Dentistry. He found that traditional Western blot uses up to 300 pieces of plastic and Wes uses two. His lab joined the university's Office of Sustainability's Green Labs Program in 2013 that encourages labs across campus to be more environmentally friendly, and won the Campus Sustainability Leadership Award in 2015.

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Sustainability with Automated Western Blotting

I'm frustrated with traditional Western blots

Traditional Westerns are as much an art form as they are a technical tool. They are rarely reproducible, they can take years to master and after you worked out the conditions they work beautifully until they don't, leaving you wondering what went wrong. Wes is fully automated, incredibly reproducible and gives you up to 25 data points in hours, not days.

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Wes Automated Western Blotting Versus Traditional Western Blotting

Simple Western Applications

Cancer &

Simple Western Instrument Cancer Research Applications


Simple Western Instrument SARS-CoV-2 Research Applications

Cell & Gene Therapy

Simple Western Instrument Cell & Gene Therapy Research Applications

C&GT Western Blotting Solutions

Cell & Gene Therapy Western Blotting Solutions


Targeted Protein Degradation

Targeted Degradation

SARS-CoV-2 Serology

SARS-CoV-2 Multi-Antigen Serology Module



Vaccine Development

Vaccines Development




Sample Required 0.3-1.2 µg 0.6-1.2 µg
Volume Required 3 µL/well 3 µL/well
Size Range Molecular weight (MW) ladder ranges from 2-440 kDa Molecular weight (MW) ladder ranges from 2-440 kDa
Sizing CV <10% <10%
Intra-assay CV <15% <15%
Inter-assay CV <20%* <20%**
Resolution (± percent difference in MW) ± 15-20% for MW <20 kDA
± 10% for MW >20 kDa
± 15-20% for MW <20 kDA
± 10% for MW >20 kDa
Quantitation CV <20% <20%
Dynamic Range 2-3 logs 3-4 logs
Sensitivity ng Low pg
Capillary 5 cm, 100 µm, 400 nL

*inter-assay CV is with system control
**percent peak area