Meet Wes, a protein characterization problem solver
Proteins are complex, but protein characterization shouldn't be. Are you struggling with methods that aren't quantitative? Are you spending all of your precious sample just to optimize your assay? Is the throughput inadequate? Are you waiting days to get your data? Watch this video to find out how Wes can be your protein characterization problem solver.
Western Blotting With Wes
He's a gel free, blot free, and hands-free protein characterization solution.
Proteins are complex, and most traditional protein characterization methods are inefficient or inadequate.
Wes is your protein characterization problem solver.
- Is your sample precious or too expensive?
- Do you need better reproducibility?
- Is time to answer too long?
- Do you need a method that is quantitative?
- Is your current method unable to handle the throughput you need?
Wes can answer all these questions and more. Protein characterization should be easy, and with Simple Western and Wes, it can be.
Making Western Blots Simple With Simple Western
Simple Western reinvents how western blots are done. Automating all steps from protein separation, immunoprobing, detection, and analysis of data.
Simple Westerns finally offer researchers an analytical tool that delivers quantifiable and reproducible results in hours versus days.
You're probably wondering exactly how Wes does simple western blots.
How Wes Performs Western Blots
The magic happens inside of a capillary. Each capillary cartridge can run either 13 or 25 capillaries — enabling throughput flexibility.
Running an immunoassay on Wes is simple.
Your sample, separation matrix, stacking matrix, antibodies, and reagents are loaded automatically from a specially designed plate.
Wes begins by aspirating the separation matrix and then the stacking matrix into each capillary, so proteins will stack tightly before they separate just like an sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Next, your protein lysate is loaded.
Capillaries are lowered making contact with running buffer and voltage is applied to enable separation by molecular weight.
Once the separation is complete, UV light immobilizes the proteins to the capillary wall.
With proteins now immobilized and the matrices cleared of the capillary, Wes starts the immunoprobing by first incubating with your primary antibody of choice followed by a secondary HRP conjugate and finally chemiluminescent substrate.
The chemiluminescent reaction gives off light that is recorded by a CCD camera in a series of images over time with fresh substrate added before each exposure.
Three hours later, you'll get quantitative, size-based data.
Protein Lysate Isolation
Interested in looking at all the proteins in your lysate?
Just leave out the antibodies and use a biotin labeling agent and Streptavidin HRP to quantify total protein.
Results are reported by electropherogram and blot view.
Stop waiting three days for something that can take three hours.
Win the race to discovery with Wes.
Learn more about Wes by ProteinSimple, a Bio-Techne brand, and western blotting by visiting the Wes instrument page.